Anti-MMS2 antibody (ab155007)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MMS2 antibody
See all MMS2 primary antibodies -
Description
Rabbit polyclonal to MMS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 1-145 of Human MMS2 (Q15819).
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Positive control
- HL-60 and BCL-1 whole cell lysate. HBL435 xenograft tissue. C2C12 xenograft tissue.
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General notes
Keep as concentrated solution.This product was previously labelled as Ube2v2, MMS2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. -
Tissue specificity
Detected in placenta, colon, liver and skin. Detected at very low levels in most tissues. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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Database links
- Entrez Gene: 286803 Cow
- Entrez Gene: 608470 Dog
- Entrez Gene: 7336 Human
- Entrez Gene: 100503412 Mouse
- Entrez Gene: 70620 Mouse
- Entrez Gene: 100627960 Pig
- Entrez Gene: 287927 Rat
- Entrez Gene: 380089 Xenopus laevis
see all -
Alternative names
- 1 alpha 25 dihydroxyvitamin D3 inducible antibody
- DDVit 1 antibody
- DDVIT1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS2 antibody (ab155007)Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labelling MMS2 with ab155007 at 1/500 dilution. -
Western blot - Anti-MMS2 antibody (ab155007)Anti-MMS2 antibody (ab155007) at 1/1000 dilution + HL-60 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Western blot - Anti-MMS2 antibody (ab155007)Anti-MMS2 antibody (ab155007) at 1/1000 dilution + BCL-1 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS2 antibody (ab155007)Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, labelling MMS2 with ab155007 at 1/500 dilution.
References
ab155007 has not yet been referenced specifically in any publications.
