Anti-MMS2 antibody (ab155007)
Key features and details
- Rabbit polyclonal to MMS2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MMS2 antibody
See all MMS2 primary antibodies -
Description
Rabbit polyclonal to MMS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-145 of Human MMS2 (Q15819).
-
Positive control
- HL-60 and BCL-1 whole cell lysate. HBL435 xenograft tissue. C2C12 xenograft tissue.
-
General notes
Keep as concentrated solution.This product was previously labelled as Ube2v2, MMS2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. -
Tissue specificity
Detected in placenta, colon, liver and skin. Detected at very low levels in most tissues. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
-
Database links
- Entrez Gene: 286803 Cow
- Entrez Gene: 608470 Dog
- Entrez Gene: 7336 Human
- Entrez Gene: 100503412 Mouse
- Entrez Gene: 70620 Mouse
- Entrez Gene: 100627960 Pig
- Entrez Gene: 287927 Rat
- Entrez Gene: 380089 Xenopus laevis
see all -
Alternative names
- 1 alpha 25 dihydroxyvitamin D3 inducible antibody
- DDVit 1 antibody
- DDVIT1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS2 antibody (ab155007)Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labelling MMS2 with ab155007 at 1/500 dilution.
-
Western blot - Anti-MMS2 antibody (ab155007)Anti-MMS2 antibody (ab155007) at 1/1000 dilution + HL-60 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Western blot - Anti-MMS2 antibody (ab155007)Anti-MMS2 antibody (ab155007) at 1/1000 dilution + BCL-1 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS2 antibody (ab155007)Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, labelling MMS2 with ab155007 at 1/500 dilution.
Protocols
References (0)
ab155007 has not yet been referenced specifically in any publications.
