Overview

  • Product name

    Anti-MMS21 antibody [215C]
    See all MMS21 primary antibodies
  • Description

    Mouse monoclonal [215C] to MMS21
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human colon, breast and breast carcinoma tissue. WB: HEK-293T, Jurkat, A431 and HeLa whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab241564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the NSE2 family.
    Contains 1 SP-RING-type zinc finger.
  • Post-translational
    modifications

    Sumoylated, possibly via autosumoylation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C8orf36 antibody
    • E3 SUMO-protein ligase NSE2 antibody
    • hMMS21 antibody
    • hNSE2 antibody
    • methyl methanesulfonate sensitivity gene 21 antibody
    • MMS21 antibody
    • MMS21 homolog antibody
    • non-SMC element 2 antibody
    • Non-SMC element 2 homolog antibody
    • Non-structural maintenance of chromosomes element 2 homolog antibody
    • NSE2 antibody
    • NSE2_HUMAN antibody
    • Nsmce2 antibody
    see all

Images

  • All lanes : Anti-MMS21 antibody [215C] (ab241564) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MMS21 with ab241564 at 1/100 dilution, followed by ready to use secondary antibody. Nuclear staining on human colon tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue labeling MMS21 with ab241564 at 1/100 dilution, followed by ready to use secondary antibody. Nuclear staining on human breast tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MMS21 with ab241564 at 1/100 dilution, followed by ready to use secondary antibody. Nuclear staining on human breast carcinoma tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

References

ab241564 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241564.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up