Key features and details
- Mouse polyclonal to MMS21
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMS21 antibody
See all MMS21 primary antibodies
DescriptionMouse polyclonal to MMS21
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Full length protein corresponding to Human MMS21 aa 1-247.
MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVE SQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDL KLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADG TEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIES RQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE
- Recombinant Human MMS21 protein (ab132298) can be used as a positive control in WB. MMS21 transfected 293T cell lysate; HeLa cells
Previously labelled as NSE2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the NSE2 family.
Contains 1 SP-RING-type zinc finger.
modificationsSumoylated, possibly via autosumoylation.
- Information by UniProt
- C8orf36 antibody
- E3 SUMO-protein ligase NSE2 antibody
- hMMS21 antibody
All lanes : Anti-MMS21 antibody (ab169355) at 1 µg/ml
Lane 1 : MMS21 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 28 kDa
Immunofluorescent analysis of HeLa cells labeling MMS21with ab169355 at 10 µg/ml.
ab169355 has not yet been referenced specifically in any publications.