Key features and details
- Rabbit polyclonal to MMS21
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMS21 antibody
See all MMS21 primary antibodies
DescriptionRabbit polyclonal to MMS21
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human MMS21 aa 197-247. The exact sequence is proprietary. (NP_775956.1).
Database link: Q96MF7
- IP: HeLa whole cell lysate.
ab241347 has not performed satisfactorily when used for WB of MMS21 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MMS21.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241347 was affinity purified using an epitope specific to MMS21 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the NSE2 family.
Contains 1 SP-RING-type zinc finger.
modificationsSumoylated, possibly via autosumoylation.
- Information by UniProt
- C8orf36 antibody
- E3 SUMO-protein ligase NSE2 antibody
- hMMS21 antibody
MMS21 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded). ab241347 used for IP at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241347 at 1 µg/ml.
Lane 1: ab241347 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241347 has not yet been referenced specifically in any publications.