Overview

  • Product name

  • Description

    Rabbit polyclonal to MMS21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MMS21 aa 197-247. The exact sequence is proprietary. (NP_775956.1).
    Database link: Q96MF7

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    ab241347 has not performed satisfactorily when used for WB of MMS21 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MMS21.

Properties

Applications

Our Abpromise guarantee covers the use of ab241347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
    • Pathway

      Protein modification; protein sumoylation.
    • Sequence similarities

      Belongs to the NSE2 family.
      Contains 1 SP-RING-type zinc finger.
    • Post-translational
      modifications

      Sumoylated, possibly via autosumoylation.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C8orf36 antibody
      • E3 SUMO-protein ligase NSE2 antibody
      • hMMS21 antibody
      • hNSE2 antibody
      • methyl methanesulfonate sensitivity gene 21 antibody
      • MMS21 antibody
      • MMS21 homolog antibody
      • non-SMC element 2 antibody
      • Non-SMC element 2 homolog antibody
      • Non-structural maintenance of chromosomes element 2 homolog antibody
      • NSE2 antibody
      • NSE2_HUMAN antibody
      • Nsmce2 antibody
      see all

    Images

    • MMS21 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded). ab241347 used for IP at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241347 at 1 µg/ml.

      Lane 1: ab241347 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 3 minutes.

    References

    ab241347 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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