Key features and details
- Rabbit polyclonal to MMS21
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMS21 antibody
See all MMS21 primary antibodies
DescriptionRabbit polyclonal to MMS21
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MMS21 aa 125-175. The exact sequence is proprietary. (NP_775956.1)
Database link: Q96MF7
- IP: HeLa whole cell lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to MMS21 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the NSE2 family.
Contains 1 SP-RING-type zinc finger.
modificationsSumoylated, possibly via autosumoylation.
- Information by UniProt
- C8orf36 antibody
- E3 SUMO-protein ligase NSE2 antibody
- hMMS21 antibody
MMS21 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241355 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different MMS21 antibody at 1 µg/ml.
Lane 1: ab241355 (batch 1) IP in HeLa whole cell lysate.
Lane 2: ab241355 (batch 2) IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 75 seconds.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241355 has not yet been referenced specifically in any publications.