Overview

  • Product name

  • Description

    Rabbit polyclonal to MMS21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MMS21 aa 125-175. The exact sequence is proprietary. (NP_775956.1)
    Database link: Q96MF7

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
    • Pathway

      Protein modification; protein sumoylation.
    • Sequence similarities

      Belongs to the NSE2 family.
      Contains 1 SP-RING-type zinc finger.
    • Post-translational
      modifications

      Sumoylated, possibly via autosumoylation.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C8orf36 antibody
      • E3 SUMO-protein ligase NSE2 antibody
      • hMMS21 antibody
      • hNSE2 antibody
      • methyl methanesulfonate sensitivity gene 21 antibody
      • MMS21 antibody
      • MMS21 homolog antibody
      • non-SMC element 2 antibody
      • Non-SMC element 2 homolog antibody
      • Non-structural maintenance of chromosomes element 2 homolog antibody
      • NSE2 antibody
      • NSE2_HUMAN antibody
      • Nsmce2 antibody
      see all

    Images

    • MMS21 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241355 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different MMS21 antibody at 1 µg/ml.

      Lane 1: ab241355 (batch 1) IP in HeLa whole cell lysate.

      Lane 2: ab241355 (batch 2) IP in HeLa whole cell lysate.

      Lane 3: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 75 seconds.

      Lysates prepared in NETN buffer.

    References

    ab241355 has not yet been referenced specifically in any publications.

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