Anti-MMS21 antibody (ab64683)
Key features and details
- Rabbit polyclonal to MMS21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-MMS21 antibody
See all MMS21 primary antibodies -
Description
Rabbit polyclonal to MMS21 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human MMS21 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94387) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: Caco2; HepG2; A431.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 28 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the NSE2 family.
Contains 1 SP-RING-type zinc finger. -
Post-translational
modificationsSumoylated, possibly via autosumoylation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 519974 Cow
- Entrez Gene: 286053 Human
- Entrez Gene: 68501 Mouse
- Entrez Gene: 299957 Rat
- SwissProt: Q32KY9 Cow
- SwissProt: Q96MF7 Human
- SwissProt: Q91VT1 Mouse
- SwissProt: Q4V8A0 Rat
see all -
Alternative names
- C8orf36 antibody
- E3 SUMO-protein ligase NSE2 antibody
- hMMS21 antibody
see all
Images
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All lanes : Anti-MMS21 antibody (ab64683) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesHuman E3 SUMO-protein ligase MMS21 contains a potential sumoylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab64683 has not yet been referenced specifically in any publications.