Recombinant
RabMAb

Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)

Overview

  • Product name
    Anti-MMS22L (C6ORF167) antibody [EPR13654]
    See all MMS22L (C6ORF167) primary antibodies
  • Description
    Rabbit Monoclonal [EPR13654] to MMS22L (C6ORF167)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human MMS22L (C6ORF167) aa 1100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6ZRQ5

  • Positive control
    • HeLa, K562 and HCT-116 whole cell lysate; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/20.

Target

  • Function
    Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA.
  • Sequence similarities
    Belongs to the MMS22 family. MMS22L subfamily.
  • Cellular localization
    Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6orf167 antibody
    • C6orf167 chromosome 6 open reading frame 167 antibody
    • chromosome 6 open reading frame 167 antibody
    • dJ39B17.2 antibody
    • DKFZp686C20164 antibody
    • DKFZp781C2113 antibody
    • FLJ46180 antibody
    • homologous to yeast Mms22 antibody
    • KIAA1900 antibody
    • Methyl methanesulfonate-sensitivity protein 22-like antibody
    • MMS22 like, DNA repair protein antibody
    • Mms22-like protein antibody
    • MMS22_HUMAN antibody
    • MMS22L antibody
    • OTTHUMP00000221009 antibody
    • Protein MMS22-like antibody
    • Uncharacterized protein C6orf167 antibody
    see all

Images

  • All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : HCT-116 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 142 kDa

  • Flow Cytometric analysis of K562 cells labeling MMS22L with ab181047 at a 1/20 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181047 at 1/20 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab181047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary Gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8.5
Permeabilization
No
Specification
Mammary Gland
Blocking step
Super Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
10% Formalin
Username

Abcam user community

Verified customer

Submitted Feb 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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