Recombinant
RabMAb

Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)

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Overview

  • Product name
    Anti-MMS22L (C6ORF167) antibody [EPR13654]
    See all MMS22L (C6ORF167) primary antibodies
  • Description
    Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human MMS22L (C6ORF167) aa 1100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6ZRQ5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa, K562 and HCT-116 whole cell lysate; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/20.

Target

  • Function
    Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA.
  • Sequence similarities
    Belongs to the MMS22 family. MMS22L subfamily.
  • Cellular localization
    Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks.
  • Information by UniProt
  • Database links
    • Alternative names
      • C6orf167 antibody
      • C6orf167 chromosome 6 open reading frame 167 antibody
      • chromosome 6 open reading frame 167 antibody
      • dJ39B17.2 antibody
      • DKFZp686C20164 antibody
      • DKFZp781C2113 antibody
      • FLJ46180 antibody
      • homologous to yeast Mms22 antibody
      • KIAA1900 antibody
      • Methyl methanesulfonate-sensitivity protein 22-like antibody
      • MMS22 like, DNA repair protein antibody
      • Mms22-like protein antibody
      • MMS22_HUMAN antibody
      • MMS22L antibody
      • OTTHUMP00000221009 antibody
      • Protein MMS22-like antibody
      • Uncharacterized protein C6orf167 antibody
      see all

    Images

    • Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate
      Lane 3 : HCT-116 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 142 kDa

    • Flow Cytometry - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      Flow Cytometry - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)

      Flow Cytometric analysis of K562 cells labeling MMS22L with ab181047 at a 1/20 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

    • Immunoprecipitation - Anti-MMS22L (C6ORF167) [EPR13654] antibody (ab181047)
      Immunoprecipitation - Anti-MMS22L (C6ORF167) [EPR13654] antibody (ab181047)

      Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181047 at 1/20 dilution.
      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    References

    ab181047 has not yet been referenced specifically in any publications.

    Publishing research using ab181047? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMS22L (C6ORF167) antibody [EPR13654]

     Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mammary Gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH 8.5
    Permeabilization
    No
    Specification
    Mammary Gland
    Blocking step
    Super Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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