Recombinant Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
![Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.com/ps/products/181/ab181047/Images/ab181047-215203-anti-mms22l-c6orf167-antibody-epr13654-western-blot.jpg "Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167)
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MMS22L (C6ORF167) antibody [EPR13654]
See all MMS22L (C6ORF167) primary antibodies -
Description
Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human MMS22L (C6ORF167) aa 1100 to the C-terminus. The exact sequence is proprietary.
Database link: Q6ZRQ5 -
Positive control
- HeLa, K562 and HCT-116 whole cell lysate; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13654 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab181047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa). | |
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IP | 1/20. |
Target
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Function
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. -
Sequence similarities
Belongs to the MMS22 family. MMS22L subfamily. -
Cellular localization
Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks. - Information by UniProt
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Database links
- Entrez Gene: 253714 Human
- Entrez Gene: 212377 Mouse
- Omim: 615614 Human
- SwissProt: Q6ZRQ5 Human
- SwissProt: B1AUR6 Mouse
- Unigene: 444292 Human
- Unigene: 120919 Mouse
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Alternative names
- C6orf167 antibody
- C6orf167 chromosome 6 open reading frame 167 antibody
- chromosome 6 open reading frame 167 antibody
see all
Images
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Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : HCT-116 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 142 kDa -
![Flow Cytometry - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.com/ps/products/181/ab181047/Images/ab181047-215202-anti-mms22l-c6orf167-antibody-epr13654-flow-cytometry.jpg)
Flow Cytometry - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)Flow Cytometric analysis of K562 cells labeling MMS22L with ab181047 at a 1/20 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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![Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.com/ps/products/181/ab181047/Images/ab181047-215201-anti-mms22l-c6orf167-antibody-epr13654-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181047 at 1/20 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
![Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.com/ps/products/181/ab181047/Images/ab181047-4-benefits-of-recombinant-antibodies.png)
Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
Protocols
Datasheets and documents
Certificate of Compliance
References (2)
ab181047 has been referenced in 2 publications.
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Huang TH et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807
