Recombinant
RabMAb

Recombinant Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)

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Overview

  • Product name

    Anti-MMS22L (C6ORF167) antibody [EPR13654]
    See all MMS22L (C6ORF167) primary antibodies

  • Description

    Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human MMS22L (C6ORF167) aa 1100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6ZRQ5

  • Positive control

    • HeLa, K562 and HCT-116 whole cell lysate; K562 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/20.

Target

  • Function

    Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA.

  • Sequence similarities

    Belongs to the MMS22 family. MMS22L subfamily.

  • Cellular localization

    Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks.
  • Information by UniProt

  • Database links

    • Alternative names

      • C6orf167 antibody
      • C6orf167 chromosome 6 open reading frame 167 antibody
      • chromosome 6 open reading frame 167 antibody
      • dJ39B17.2 antibody
      • DKFZp686C20164 antibody
      • DKFZp781C2113 antibody
      • FLJ46180 antibody
      • homologous to yeast Mms22 antibody
      • KIAA1900 antibody
      • Methyl methanesulfonate-sensitivity protein 22-like antibody
      • MMS22 like, DNA repair protein antibody
      • Mms22-like protein antibody
      • MMS22_HUMAN antibody
      • MMS22L antibody
      • OTTHUMP00000221009 antibody
      • Protein MMS22-like antibody
      • Uncharacterized protein C6orf167 antibody
      see all

    Images

    • Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate
      Lane 3 : HCT-116 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 142 kDa

    • Flow Cytometry - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      Flow Cytometry - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)

      Flow Cytometric analysis of K562 cells labeling MMS22L with ab181047 at a 1/20 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

    • Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
      Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)

      Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181047 at 1/20 dilution.
      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    References

    This product has been referenced in:

    • Sun H  et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). Read more (PubMed: 30779731) »
    See 1 Publication for this product

    Publishing research using ab181047? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMS22L (C6ORF167) antibody [EPR13654]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mammary Gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH 8.5
    Permeabilization
    No
    Specification
    Mammary Gland
    Blocking step
    Super Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2016

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