The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA.
Belongs to the MMS22 family. MMS22L subfamily.
Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks.
Flow Cytometric analysis of K562 cells labeling MMS22L with ab181047 at a 1/20 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181047 at 1/20 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.