Product nameAnti-MMS22L (C6ORF167) antibody [EPR13654]
See all MMS22L (C6ORF167) primary antibodies
DescriptionRabbit monoclonal [EPR13654] to MMS22L (C6ORF167)
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Human
- HeLa, K562 and HCT-116 whole cell lysate; K562 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionComponent of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA.
Sequence similaritiesBelongs to the MMS22 family. MMS22L subfamily.
Cellular localizationNucleus. Localizes to DNA damage sites, accumulates at stressed replication forks.
- Information by UniProt
- C6orf167 antibody
- C6orf167 chromosome 6 open reading frame 167 antibody
- chromosome 6 open reading frame 167 antibody
All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : HCT-116 whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 142 kDa
Flow Cytometric analysis of K562 cells labeling MMS22L with ab181047 at a 1/20 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181047 at 1/20 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
ab181047 has not yet been referenced specifically in any publications.