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    mnat1-antibody-ab175398.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Cdk Regulators
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Anti-MNAT1 antibody (ab175398)

  • Datasheet
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Western blot - Anti-MNAT1 antibody (ab175398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNAT1 antibody (ab175398)
  • Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody (ab175398)

Key features and details

  • Rabbit polyclonal to MNAT1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MNAT1 antibody
    See all MNAT1 primary antibodies
  • Description

    Rabbit polyclonal to MNAT1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human MNAT1 aa 1-309.
    Sequence:

    MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECG TPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLE EVEEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEA LEVERQENEQRRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQ HKDRSTQLEMQLEKPKPVKPVTFSTGIKMGQHISLAPIHKLEEALYEYQP LQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGGYTSSLACHRALQDA FSGLFWQPS


    Database link: P51948
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • A431 and Hela cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdk Regulators
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIIH
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdk Regulators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human MNAT1 protein (ab204195)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Rat
WB
Human
Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/500 - 1/2000. Predicted molecular weight: 36 kDa.
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/500 - 1/2000. Predicted molecular weight: 36 kDa.
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
  • Tissue specificity

    Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P51948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4331 Human
    • Entrez Gene: 266713 Rat
    • Omim: 602659 Human
    • SwissProt: P51948 Human
    • Unigene: 509523 Human
    • Unigene: 15168 Rat
    • Alternative names

      • MNAT 1 antibody
      • CAP35 antibody
      • CDK activating kinase assembly factor MAT1 antibody
      • CDK-activating kinase assembly factor MAT1 antibody
      • CDK7/cyclin H assembly factor antibody
      • CDK7/cyclin-H assembly factor antibody
      • Cyclin G1 interacting protein antibody
      • Cyclin-G1-interacting protein antibody
      • MAT1 antibody
      • MAT1_HUMAN antibody
      • Menage a trois 1 (CAK assembly factor) antibody
      • Menage a trois antibody
      • Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) antibody
      • MNAT CDK activating kinase assembly factor 1 antibody
      • Mnat1 antibody
      • p35 antibody
      • p36 antibody
      • RING finger protein 66 antibody
      • RING finger protein MAT1 antibody
      • RNF66 antibody
      • TFB3 antibody
      see all

    Images

    • Western blot - Anti-MNAT1 antibody (ab175398)
      Western blot - Anti-MNAT1 antibody (ab175398)
      All lanes : Anti-MNAT1 antibody (ab175398) at 1/500 dilution

      Lane 1 : A431 cell extract
      Lane 2 : Hela cell extract

      Predicted band size: 36 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNAT1 antibody (ab175398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNAT1 antibody (ab175398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling MNAT1 with ab175398 at 1/200. Magnification: 200x.
    • Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody (ab175398)
      Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody (ab175398)
      Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175398. Blue DAPI for nuclear staining.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab175398? Please let us know so that we can cite the reference in this datasheet.

    ab175398 has not yet been referenced specifically in any publications.

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