The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/600 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Use at an assay dependent concentration.
Is unsuitable for IHC-Fr.
May act as a transcriptional activator/repressor in the myeloid lineage. Plays a role in the granulocyte/monocyte cell-specific response to interferon. Stimulates the DNA binding of the transcriptional repressor protein YY1.
Expressed constitutively in cells of the myeloid lineage. Found in promyelocyte stage cells as well as in all other stage cells including peripheral blood monocytes and granulocytes. Also appear in myeloblast cells in some cases of acute myeloid Leukemia.
Western blot - Anti-MNDA antibody [253A] (ab188566)
All lanes : Anti-MNDA antibody [253A] (ab188566)
Lane 1 : Hek-v5-MNDA cell line (Positive) Lane 2 : Hek-v5-CD1D cell line (Negative control) Lane 3 : HL60 cell line (Negative) Lane 4 : K562 cell line (Negative) Lane 5 : Z138 cell line (Positive) Lane 6 : MINO cell line (Positive) Lane 7 : YT cell line (Negative) Lane 8 : MT-2 cell line (Positive)