• Product name

    Anti-MNK2 antibody
  • Description

    Rabbit polyclonal to MNK2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (SDFGLQCSAR PDMPASQPID IPDAKKRGKK KKRGRATDSF SGRFEDVYQL) of human MNK2 (NP_951009).

  • Positive control

    • 721_B cell lysate



Our Abpromise guarantee covers the use of ab84345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.


  • Function

    May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificity

    Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational

    Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor kinase 7 antibody
    • GPRK7 antibody
    • MAP kinase interacting serine threonine kinase 2 antibody
    • MAP kinase interacting serine threonine kinase antibody
    • MAP kinase interacting serine threonine protein kinase 2 antibody
    • MAP kinase signal integrating kinase 2 antibody
    • MAP kinase signal-integrating kinase 2 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 2 antibody
    • MAPK interacting kinase 2 antibody
    • MAPK signal integrating kinase 2 antibody
    • Mitogen activated protein kinase interacting serine threonine kinase 2 antibody
    • Mknk2 antibody
    • MKNK2_HUMAN antibody
    • Mnk2 antibody
    • Putative map kinase interacting kinase antibody
    see all


  • ab84345 at 5 µg/ml staining MNK2 in Human Placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-MNK2 antibody (ab84345) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • ICC/IF image of ab84345 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84345, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab84345 at 5 µg/ml staining MNK2 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • IHC image of ab84345 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84345, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-MNK2 antibody (ab84345) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 10 µg
    Lane 4 : Zebrafish liver homogenate at 10 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 10 µg
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

    Exposure time: 5 minutes


This product has been referenced in:

  • Hu J  et al. Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice. Biol Reprod 98:102-114 (2018). Read more (PubMed: 29161344) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for contacting Abcam.The immunogen sequence for ab84345 is available on the public website and so I blasted this sequence against the human genome and it showed that the immunogen had 100% homology to both isoforms 2a and 2b of MNK2.As to the HEK cell image on the website, I believe that the staining is of endogenous MNK2, as I cannot find any reference to this these cells being transfected with any plasmids.I hope that this information is useful and if there is anything else I can help you with, please let me know.Happy New Year

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