Key features and details
- Rabbit polyclonal to mnt
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-mnt antibody
See all mnt primary antibodies
DescriptionRabbit polyclonal to mnt
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to Human mnt aa 1-50 (N terminal).
Database link: NP_064706.1
- HeLa, 293T and Jurkat whole cell lysates.
Previously labelled as MAX binding protein.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab129466 is affinity purified using an epitope specific to mnt immobilized on solid support.
Our Abpromise guarantee covers the use of ab129466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 62 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- bHLHd3 antibody
- Class D basic helix-loop-helix protein 3 antibody
- MAD6 antibody
All lanes : Anti-mnt antibody (ab129466) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Predicted band size: 62 kDa
Detection of mnt by Western Blot of Immunoprecipitates.
ab129466 at 1µg/ml staining mnt in HeLa whole cell lysate immunoprecipitated using ab129466 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab129466 has not yet been referenced specifically in any publications.