Key features and details
- Rabbit polyclonal to Mob1A
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Mob1A antibody
See all Mob1A primary antibodies
DescriptionRabbit polyclonal to Mob1A
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
Recombinant full length protein corresponding to Human Mob1A aa 2-216.
MSFLFSSRSSKTFKPKKNIPEGSHQYELLKHAEATLGSGNLRQAVMLPEG EDLNEWIAVNTVDFFNQINMLYGTITEFCTEASCPVMSAGPRYEYHWADG TNIKKPIKCSAPKYIDYLMTWVQDQLDDETLFPSKIGVPFPKNFMSVAKT ILKRLFRVYAHIYHQHFDSVMQLQEEAHLNTSFKHFIFFVQEFNLIDRRE LAPLQELIEKLGSKDR
Database link: Q9H8S9
- WB: Jurkat and K562 whole cell lysate; Mouse small intestine tissue lysate. IHC-P: Human small intestine and breast cancer tissue. ICC/IF: HepG2 cells. IP: Jurkat whole cell lysate.
This product was previously labelled as MOBK1B
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/1000.|
|ICC/IF||1/200 - 1/500.|
FunctionActivator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L.
Tissue specificityAdrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver.
Sequence similaritiesBelongs to the MOB1/phocein family.
- Information by UniProt
- B1, Mps One Binder kinase activator like 1B (yeast) antibody
- C2orf6 antibody
- Chromosome 2 open reading frame 6 antibody
All lanes : Anti-Mob1A antibody (ab236969) at 1/200 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse small intestine tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Paraffin-embedded human small intestine tissue stained for Mob1A using ab236969 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mob1A (Green) using ab236969 at 1/333 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Anti-Mob1A antibody (ab236969) at 1/200 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Mob1A was immunoprecipitated from 500 μg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 1: Rabbit control IgG instead of ab236969 in Jurkat cell lysate.
Lane 2: ab236969 IP in Jurkat cell lysate.
Lane 3: Jurkat whole cell lysate (input).
Paraffin-embedded human breast cancer tissue stained for Mob1A using ab236969 at 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236969 has not yet been referenced specifically in any publications.