Key features and details
- Rabbit polyclonal to MOCS1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MOCS1 antibody
See all MOCS1 primary antibodies
DescriptionRabbit polyclonal to MOCS1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: RT4 whole cells; IHC-P: Human smooth muscle tissue; ICC: Caco-2 whole cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionIsoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of a guanosine derivative to precursor Z during molybdenum cofactor biosynthesis.
Tissue specificityIsoform MOCS1A and isoform 2 are widely expressed.
PathwayCofactor biosynthesis; molybdopterin biosynthesis.
Involvement in diseaseDefects in MOCS1 are the cause of molybdenum cofactor deficiency type A (MOCOD type A) [MIM:252150]; an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
Sequence similaritiesIn the C-terminal section; belongs to the moaC family.
In the N-terminal section; belongs to the moaA/nifB/pqqE family.
modificationsIsoform MOCS1A, isoform 2 and isoform 3 are probably thiocarboxylated at their C-terminus. Thiocarboxylation probably plays a central role in molybdenum cofactor biosynthesis, since mutagenesis of the last 2 Gly residues of isoform MOCS1A abolishes the catalytic activity of the enzyme. Thiocarboxylation is absent in isoform MOCS1B, which lacks the C-terminal Gly residue.
- Information by UniProt
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Anti-MOCS1 antibody (ab126560) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysate
Immunohistochemical analysis of human smooth muscle tissue labeling MOCS1 in the cytoplasm of smooth muscle cells with ab126560 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis of Caco-2 (Human colorectal adenocarcinoma cell line) whole cells labeling MOCS1 in the plasma membrane with ab126560 at 2 µg/ml.
ab126560 has not yet been referenced specifically in any publications.