Key features and details
- Rabbit polyclonal to MOCS2
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MOCS2 antibody
See all MOCS2 primary antibodies
DescriptionRabbit polyclonal to MOCS2
Tested applicationsSuitable for: IP, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human MOCS2 aa 1-188.
MSSLEISSSCFSLETKLPLSPPLVEDSAFEPSRKDMDEVEEKSKDVINFT AEKLSVDEVSQLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMAE NEVRKICSDIRQKWPVKHIAVFHRLGLVPVSEASIIIAVSSAHRAASLEA VSYAIDTLKAKVPIWKKEIYEESSTWKGNKECFWASNS
Database link: O96007
- WB: A549, HepG2, Jurkat and HeLa whole cell lysates. IHC-P: Human kidney and adrenal gland tissues. IP: Jurkat whole cell lysate. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 21, 49 kDa (predicted molecular weight: 21 kDa).|
|ICC/IF||1/50 - 1/200.|
RelevanceThe large (MOCS2B) and small (MOCS2A) subunits of molybdopterin synthase are both encoded from the MOCS2 gene by overlapping open reading frames. MOCS2B is the catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. It acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group.
- MCBPE antibody
- MOCO1 antibody
- MOCO1 B antibody
All lanes : Anti-MOCS2 antibody (ab230229) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21,49 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for MOCS2 using ab230229 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for MOCS2 using ab230229 at 1/100 dilution in immunohistochemical analysis.
MOCS2 was immunoprecipitated from 500 µg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 µg ab230229.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in Jurkat whole cell lysate.
Lane 2: ab230229 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MOCS2 (green) using ab230229 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230229 has not yet been referenced specifically in any publications.