Key features and details
- Rabbit polyclonal to MOCS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MOCS3 antibody
See all MOCS3 primary antibodies
DescriptionRabbit polyclonal to MOCS3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
Recombinant fragment, corresponding to a region within amino acids 220-460 of Human MOCS3 (Uniprot ID: O95396).
- A549, HeLa, HepG2 and HCT116 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
FunctionPlays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
PathwaytRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Cofactor biosynthesis; molybdopterin biosynthesis.
Sequence similaritiesIn the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily.
Contains 1 rhodanese domain.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Adenylyltransferase MOCS3 antibody
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ab154107 has been referenced in 1 publication.
- Kim KS et al. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet 27:837-852 (2018). PubMed: 29309586