Anti-MOCS3 antibody (ab229827)
Key features and details
- Rabbit polyclonal to MOCS3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MOCS3 antibody
See all MOCS3 primary antibodies -
Description
Rabbit polyclonal to MOCS3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MOCS3 aa 250 to the C-terminus.
Database link: O95396 -
Positive control
- WB: A549, HepG2 and Jurkat whole cell lysates. IHC-P: Human liver and brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. -
Pathway
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Cofactor biosynthesis; molybdopterin biosynthesis. -
Sequence similarities
In the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily.
Contains 1 rhodanese domain. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 27304 Human
- GenBank: BC015939 Human
- Omim: 609277 Human
- SwissProt: O95396 Human
- Unigene: 159410 Human
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Alternative names
- Adenylyltransferase MOCS3 antibody
- dJ914P20.3 antibody
- MGC9252 antibody
see all
Images
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All lanes : Anti-MOCS3 antibody (ab229827) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Paraffin-embedded human liver tissue stained for MOCS3 using ab229827 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human brain tissue stained for MOCS3 using ab229827 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229827 has not yet been referenced specifically in any publications.