Product nameAnti-MOCS3 antibody
See all MOCS3 primary antibodies
DescriptionRabbit polyclonal to MOCS3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MOCS3 aa 271-460.
YSGSLLLFDALRGHFRSIRLRSRRLDCAACGERPTVTDLLDYEAFCGSSA TDKCRSLQLLSPEERVSVTDYKRLLDSGAFHLLLDVRPQVEVDICRLPHA LHIPLKHLERRDAESLKLLKEAIWEEKQGTQEGAAVPIYVICKLGNDSQK AVKILQSLSAAQELDPLTVRDVVGGLMAWAAKIDGTFPQY
Database link: O95396
- WB: A549, HepG2 and Jurkat whole cell lysates. IHC-P: Human liver tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionPlays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
PathwaytRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Cofactor biosynthesis; molybdopterin biosynthesis.
Sequence similaritiesIn the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily.
Contains 1 rhodanese domain.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Adenylyltransferase MOCS3 antibody
- dJ914P20.3 antibody
- MGC9252 antibody
All lanes : Anti-MOCS3 antibody (ab229936) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraffin-embedded human liver tissue stained for MOCS3 using ab229936 at 1/100 dilution in immunohistochemical analysis.
ab229936 has not yet been referenced specifically in any publications.