Overview

  • Product name
  • Description
    Goat polyclonal to Moesin
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    C-NARDESKKTAND

    , corresponding to internal sequence amino acids 531-542 of Human Moesin

  • Positive control
    • Human Spleen lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA:Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probably involved in connections of major cytoskeletal structures to the plasma membrane.
    • Tissue specificity
      In all tissues and cultured cells studied.
    • Sequence similarities
      Contains 1 FERM domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
    • Cellular localization
      Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis luminal protein 70 antibody
      • HEL70 antibody
      • Membrane organizing extension spike protein antibody
      • Membrane-organizing extension spike protein antibody
      • MOES_HUMAN antibody
      • Moesin antibody
      • Moesin/anaplastic lymphoma kinase fusion protein antibody
      • Msn antibody
      • MSN/ALK fusion antibody
      see all

    Images

    • Anti-Moesin antibody (ab50430) at 1 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).

      Predicted band size: 68 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    This product has been referenced in:
    • Rørvig S  et al. Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors. J Leukoc Biol 94:711-21 (2013). Read more (PubMed: 23650620) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab50430.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up