Recombinant Anti-Moesin antibody [EPR2428(2)] (ab151542)
![Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/ab151542-297792-anti-moesin-antibody-epr24282-western-blot.jpg "Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2428(2)] to Moesin
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Moesin antibody [EPR2428(2)]
See all Moesin primary antibodies -
Description
Rabbit monoclonal [EPR2428(2)] to Moesin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Moesin aa 400-500. The exact sequence is proprietary.
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Positive control
- Raji, Jurkat and HeLa whole cell lysate (ab150035); Human breast carcinoma tissue; Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2428(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
Tissue specificity
In all tissues and cultured cells studied. -
Sequence similarities
Contains 1 FERM domain. -
Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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Database links
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: P26038 Human
- SwissProt: P26041 Mouse
- SwissProt: O35763 Rat
- Unigene: 87752 Human
see all -
Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Images
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Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Moesin knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab151542 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab151542 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in Moesin knockout cells. Wild-type and Moesin knockout samples were subjected to SDS-PAGE. Ab151542 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/ab151542-158531-anti-moesin-antibody-epr24282-western-blot.jpg)
Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)All lanes : Anti-Moesin antibody [EPR2428(2)] (ab151542) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/ab151542-158530-anti-moesin-antibody-epr24282-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Moesin with ab151542 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/ab151542-158529-anti-moesin-antibody-epr24282-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)Immunofluorescent analysis of Raji cells labeling Moesin with ab151542 at 1/100 dilution. -
![Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/ab151542-5-benefits-of-recombinant-antibodies.png)
Anti-Moesin antibody [EPR2428(2)] (ab151542)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151542 has been referenced in 1 publication.
- Mu L et al. A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programing. Nat Commun 9:4259 (2018). PubMed: 30323235