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    moesin-antibody-epr24282-ab151542.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Talin / Moesin
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Moesin antibody [EPR2428(2)] (ab151542)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (1)

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Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
  • Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)
  • Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)
  • Anti-Moesin antibody [EPR2428(2)] (ab151542)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2428(2)] to Moesin
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human Moesin protein (ab124306)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Moesin antibody [EPR2428(2)]
    See all Moesin primary antibodies
  • Description

    Rabbit monoclonal [EPR2428(2)] to Moesin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Moesin aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Raji, Jurkat and HeLa whole cell lysate (ab150035); Human breast carcinoma tissue; Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2428(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Talin / Moesin

Associated products

  • Alternative Versions

    • Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Moesin protein (ab124306)
  • Related Products

    • Recombinant Human Moesin protein (ab124306)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Probably involved in connections of major cytoskeletal structures to the plasma membrane.
    • Tissue specificity

      In all tissues and cultured cells studied.
    • Sequence similarities

      Contains 1 FERM domain.
    • Post-translational
      modifications

      Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
    • Cellular localization

      Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
    • Target information above from: UniProt accession P26038 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4478 Human
      • Entrez Gene: 17698 Mouse
      • Entrez Gene: 81521 Rat
      • Omim: 309845 Human
      • SwissProt: P26038 Human
      • SwissProt: P26041 Mouse
      • SwissProt: O35763 Rat
      • Unigene: 87752 Human
      • Unigene: 138876 Mouse
      • Unigene: 2762 Rat
      see all
    • Alternative names

      • Epididymis luminal protein 70 antibody
      • HEL70 antibody
      • Membrane organizing extension spike protein antibody
      • Membrane-organizing extension spike protein antibody
      • MOES_HUMAN antibody
      • Moesin antibody
      • Moesin/anaplastic lymphoma kinase fusion protein antibody
      • Msn antibody
      • MSN/ALK fusion antibody
      see all

    Images

    • Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
      Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Moesin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab151542 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab151542 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in Moesin knockout cells. Wild-type and Moesin knockout samples were subjected to SDS-PAGE. Ab151542 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
      Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
      All lanes : Anti-Moesin antibody [EPR2428(2)] (ab151542) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 68 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Moesin with ab151542 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)
      Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)
      Immunofluorescent analysis of Raji cells labeling Moesin with ab151542 at 1/100 dilution.
    • Anti-Moesin antibody [EPR2428(2)] (ab151542)
      Anti-Moesin antibody [EPR2428(2)] (ab151542)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab151542? Please let us know so that we can cite the reference in this datasheet.

    ab151542 has been referenced in 1 publication.

    • Mu L  et al. A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programing. Nat Commun 9:4259 (2018). PubMed: 30323235

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Moesin antibody [EPR2428(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate ph 6
    Permeabilization
    No
    Specification
    brain tissue
    Blocking step
    Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Herr Dr. Markus Kipp

    Verified customer

    Submitted Aug 21 2018

    Western blot abreview for Anti-Moesin antibody [EPR2428(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount
    30 µg
    Specification
    NIH 3T3 cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

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