- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Moesin antibody [EPR2428(2)]
See all Moesin primary antibodies -
DescriptionRabbit monoclonal [EPR2428(2)] to Moesin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Moesin aa 400-500. The exact sequence is proprietary.
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Positive control
- Raji, Jurkat and HeLa cell lysates; Human breast carcinoma tissue; Raji cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR2428(2)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 68 kDa. | |
| IHC-P | 1/100 - 1/250. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. |
Target
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FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane.
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Tissue specificityIn all tissues and cultured cells studied.
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Sequence similaritiesContains 1 FERM domain.
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Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
- Information by UniProt
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Database links
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: P26038 Human
- SwissProt: P26041 Mouse
- SwissProt: O35763 Rat
- Unigene: 87752 Human
see all -
Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Images
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![Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/ab151542-297792-ab151542KOWBGDB.jpg)
Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Moesin knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab151542 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab151542 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in Moesin knockout cells. Wild-type and Moesin knockout samples were subjected to SDS-PAGE. Ab151542 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/Test-Target-DM-Primary-antibodies-ab151542-1.jpg)
Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)All lanes : Anti-Moesin antibody [EPR2428(2)] (ab151542) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/Test-Target-DM-Primary-antibodies-ab151542-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] (ab151542)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Moesin with ab151542 at 1/100 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)](https://www.abcam.com/ps/products/151/ab151542/Images/Test-Target-DM-Primary-antibodies-ab151542-4.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Moesin antibody [EPR2428(2)] (ab151542)Immunofluorescent analysis of Raji cells labeling Moesin with ab151542 at 1/100 dilution.
Protocols
References
ab151542 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"