Recombinant Anti-Moesin antibody [EPR2429(2)] (ab169789)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Moesin antibody [EPR2429(2)]
See all Moesin primary antibodies -
Description
Rabbit monoclonal [EPR2429(2)] to Moesin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide, corresponding to residues in Human Moesin (Uniprot ID: P26038)
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Positive control
- HeLa, Raji, 293T and Jurkat cell lysates; Human lung and stomach tissues; Raji cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2429(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab169789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 68 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
Tissue specificity
In all tissues and cultured cells studied. -
Sequence similarities
Contains 1 FERM domain. -
Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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Database links
- Entrez Gene: 540426 Cow
- Entrez Gene: 4478 Human
- Entrez Gene: 494458 Pig
- Omim: 309845 Human
- SwissProt: Q2HJ49 Cow
- SwissProt: P26038 Human
- SwissProt: P26042 Pig
- Unigene: 87752 Human
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Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Images
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![Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-1.jpg)
Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)All lanes : Anti-Moesin antibody [EPR2429(2)] (ab169789) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa -
![Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-2.jpg)
Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)Immunoprecipitation analysis of pellet from HeLa cell lysate, using anti-Moesin ab169789 at a 1/10 dilution. -
![Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-3.jpg)
Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)Flow cytometric analysis of permeabilized Raji cells labeling ab169789 (red) at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung tissue, labeling Moesin using ab169789 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human stomach tissue, labeling Moesin using ab169789 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
ab169789 has not yet been referenced specifically in any publications.
