Recombinant
RabMAb

Anti-Moesin antibody [EPR2429(2)] (ab169789)

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Overview

  • Product name
    Anti-Moesin antibody [EPR2429(2)]
    See all Moesin primary antibodies
  • Description
    Rabbit monoclonal [EPR2429(2)] to Moesin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Moesin (Uniprot ID: P26038)

  • Positive control
    • HeLa, Raji, 293T and Jurkat cell lysates; Human lung and stomach tissues; Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2429(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Probably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificity
    In all tissues and cultured cells studied.
  • Sequence similarities
    Contains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localization
    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt
  • Database links
    • Alternative names
      • Epididymis luminal protein 70 antibody
      • HEL70 antibody
      • Membrane organizing extension spike protein antibody
      • Membrane-organizing extension spike protein antibody
      • MOES_HUMAN antibody
      • Moesin antibody
      • Moesin/anaplastic lymphoma kinase fusion protein antibody
      • Msn antibody
      • MSN/ALK fusion antibody
      see all

    Images

    • Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      All lanes : Anti-Moesin antibody [EPR2429(2)] (ab169789) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 68 kDa

    • Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Immunoprecipitation analysis of pellet from HeLa cell lysate, using anti-Moesin ab169789 at a 1/10 dilution.
    • Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Flow cytometric analysis of permeabilized Raji cells labeling ab169789 (red) at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung tissue, labeling Moesin using ab169789 at a 1/100 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)
      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human stomach tissue, labeling Moesin using ab169789 at a 1/100 dilution.

    References

    ab169789 has not yet been referenced specifically in any publications.

    Publishing research using ab169789? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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