Recombinant Anti-Moesin antibody [EPR2429(2)] (ab169789)
![Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-1.jpg "Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2429(2)] to Moesin
- Suitable for: WB, IHC-P, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Moesin antibody [EPR2429(2)]
See all Moesin primary antibodies -
Description
Rabbit monoclonal [EPR2429(2)] to Moesin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide, corresponding to residues in Human Moesin (Uniprot ID: P26038)
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Positive control
- HeLa, Raji, 293T and Jurkat cell lysates; Human lung and stomach tissues; Raji cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2429(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab169789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 68 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
Tissue specificity
In all tissues and cultured cells studied. -
Sequence similarities
Contains 1 FERM domain. -
Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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Database links
- Entrez Gene: 540426 Cow
- Entrez Gene: 4478 Human
- Entrez Gene: 494458 Pig
- Omim: 309845 Human
- SwissProt: Q2HJ49 Cow
- SwissProt: P26038 Human
- SwissProt: P26042 Pig
- Unigene: 87752 Human
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Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Images
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Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)All lanes : Anti-Moesin antibody [EPR2429(2)] (ab169789) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa -
![Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-2.jpg)
Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)Immunoprecipitation analysis of pellet from HeLa cell lysate, using anti-Moesin ab169789 at a 1/10 dilution. -
![Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-3.jpg)
Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)Flow cytometric analysis of permeabilized Raji cells labeling ab169789 (red) at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung tissue, labeling Moesin using ab169789 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/Moesin-Primary-antibodies-ab169789-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human stomach tissue, labeling Moesin using ab169789 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Moesin antibody [EPR2429(2)] (ab169789)](https://www.abcam.com/ps/products/169/ab169789/Images/ab169789-6-benefits-of-recombinant-antibodies.png)
Anti-Moesin antibody [EPR2429(2)] (ab169789)
Protocols
Datasheets and documents
References (0)
ab169789 has not yet been referenced specifically in any publications.
