Anti-Moesin antibody [EPR2429(2)] Recombinant (ab169789)

Product name

Anti-Moesin antibody [EPR2429(2)]
See all Moesin primary antibodies
Description

Rabbit monoclonal [EPR2429(2)] to Moesin
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivity

Reacts with: Human
Predicted to work with: Cow, Pig
Immunogen

Synthetic peptide, corresponding to residues in Human Moesin (Uniprot ID: P26038)
Positive control
- HeLa, Raji, 293T and Jurkat cell lysates; Human lung and stomach tissues; Raji cells.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2429(2)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab169789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
ICC/IF		1/50 - 1/100.
IP		1/10 - 1/100.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane.
Tissue specificity

In all tissues and cultured cells studied.
Sequence similarities

Contains 1 FERM domain.
Post-translational
modifications

Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
Cellular localization

Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
Target information above from: UniProt accession P26038 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 540426 Cow
- Entrez Gene: 4478 Human
- Entrez Gene: 494458 Pig
- Omim: 309845 Human
- SwissProt: Q2HJ49 Cow
- SwissProt: P26038 Human
- SwissProt: P26042 Pig
- Unigene: 87752 Human
Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
- Membrane-organizing extension spike protein antibody
- MOES_HUMAN antibody
- Moesin antibody
- Moesin/anaplastic lymphoma kinase fusion protein antibody
- Msn antibody
- MSN/ALK fusion antibody
see all

Western blot - Anti-Moesin antibody [EPR2429(2)] (ab169789)

All lanes : Anti-Moesin antibody [EPR2429(2)] (ab169789) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : standard HRP labeled goat anti-rabbit at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 68 kDa
Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] (ab169789)

Immunoprecipitation analysis of pellet from HeLa cell lysate, using anti-Moesin ab169789 at a 1/10 dilution.
Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] (ab169789)

Flow cytometric analysis of permeabilized Raji cells labeling ab169789 (red) at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung tissue, labeling Moesin using ab169789 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] (ab169789)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human stomach tissue, labeling Moesin using ab169789 at a 1/100 dilution.

ab169789 has not yet been referenced specifically in any publications.

Publishing research using ab169789? Please let us know so that we can cite the reference in this datasheet.

Anti-Moesin antibody [EPR2429(2)] (ab169789)