Overview

  • Product name

    Anti-Moesin antibody [MSN/492] - BSA and Azide free
    See all Moesin primary antibodies
  • Description

    Mouse monoclonal [MSN/492] to Moesin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human Moesin aa 1-577.
    Sequence:

    MPKTISVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWFFGLQYQ DTKGFSTWLK LNKKVTAQDV RKESPLLFKF RAKFYPEDVS EELIQDITQR LFFLQVKEGI LNDDIYCPPE TAVLLASYAV QSKYGDFNKE VHKSGYLAGD KLLPQRVLEQ HKLNKDQWEE RIQVWHEEHR GMLREDAVLE YLKIAQDLEM YGVNYFSIKN KKGSELWLGV DALGLNIYEQ NDRLTPKIGF PWSEIRNISF NDKKFVIKPI DKKAPDFVFY APRLRINKRI LALCMGNHEL YMRRRKPDTI EVQQMKAQAR EEKHQKQMER AMLENEKKKR EMAEKEKEKI EREKEELMER LKQIEEQTKK AQQELEEQTR RALELEQERK RAQSEAEKLA KERQEAEEAK EALLQASRDQ KKTQEQLALE MAELTARISQ LEMARQKKES EAVEWQQKAQ MVQEDLEKTR AELKTAMSTP HVAEPAENEQ DEQDENGAEA SADLRADAMA KDRSEEERTT EAEKNERVQK HLKALTSELA NARDESKKTA NDMIHAENMR LGRDKYKTLR QIRQGNTKQR IDEFESM


    Database link: P26038

  • Positive control

    • Human melanoma, testicular carcinoma and placenta tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab215840 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    MSN/492
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.

Target

  • Function

    Probably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificity

    In all tissues and cultured cells studied.
  • Sequence similarities

    Contains 1 FERM domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localization

    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis luminal protein 70 antibody
    • HEL70 antibody
    • Membrane organizing extension spike protein antibody
    • Membrane-organizing extension spike protein antibody
    • MOES_HUMAN antibody
    • Moesin antibody
    • Moesin/anaplastic lymphoma kinase fusion protein antibody
    • Msn antibody
    • MSN/ALK fusion antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Moesin with ab215840 at 1 μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular carcinoma tissue labeling Moesin with ab215840 at 1 μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Moesin with ab215840 at 1 μg/ml dilution.

References

ab215840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215840.
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