Overview

  • Product name

    Anti-Moesin (phospho T558) antibody [EPR2371(2)]
    See all Moesin primary antibodies
  • Description

    Rabbit monoclonal [EPR2371(2)] to Moesin (phospho T558)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Moesin aa 550 to the C-terminus (phospho T558) (Cysteine residue). The exact sequence is proprietary.
    Database link: P26038

  • Positive control

    • Calyculin treated 293T cell lysate; Human gastric adenocarcinoma and prostate tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2371(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Probably involved in connections of major cytoskeletal structures to the plasma membrane.
    • Tissue specificity

      In all tissues and cultured cells studied.
    • Sequence similarities

      Contains 1 FERM domain.
    • Post-translational
      modifications

      Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
    • Cellular localization

      Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
    • Information by UniProt
    • Database links

    • Alternative names

      • Epididymis luminal protein 70 antibody
      • HEL70 antibody
      • Membrane organizing extension spike protein antibody
      • Membrane-organizing extension spike protein antibody
      • MOES_HUMAN antibody
      • Moesin antibody
      • Moesin/anaplastic lymphoma kinase fusion protein antibody
      • Msn antibody
      • MSN/ALK fusion antibody
      see all

    Images

    • All lanes : Anti-Moesin (phospho T558) antibody [EPR2371(2)] (ab177943) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate, treated with Calyculin

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 68 kDa

    • Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling Moesin with ab177943 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Moesin with ab177943 at 1/50 dilution.

    References

    This product has been referenced in:

    • Chen W  et al. Role of TLR4-MAP4K4 signaling pathway in models of oxygen-induced retinopathy. FASEB J N/A:fj201801086RR (2018). Read more (PubMed: 30475644) »
    • Moreno-Fortuny A  et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). Read more (PubMed: 28923978) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Caenorhabditis elegans Tissue lysate - whole (whole worm lysates)
    Gel Running Conditions
    Non-reduced Denaturing (10% T gel concentration)
    Loading amount
    30000 cells
    Specification
    whole worm lysates
    Blocking step
    Roti Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jun 20 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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