Recombinant Anti-Moesin (phospho T558) antibody [EPR2371(2)] (ab177943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2371(2)] to Moesin (phospho T558)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Moesin (phospho T558) antibody [EPR2371(2)]
See all Moesin primary antibodies -
Description
Rabbit monoclonal [EPR2371(2)] to Moesin (phospho T558) -
Host species
Rabbit -
Specificity
This antibody detects Moesin phosphorylated at threonine 558, Ezrin phosphorylated at threonine 567 and Radixin phosphorylated at threonine 564.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Calyculin treated 293T cell lysate; Human gastric adenocarcinoma and prostate tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2371(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
Tissue specificity
In all tissues and cultured cells studied. -
Sequence similarities
Contains 1 FERM domain. -
Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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Database links
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: P26038 Human
- SwissProt: P26041 Mouse
- SwissProt: O35763 Rat
- Unigene: 87752 Human
see all -
Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Images
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All lanes : Anti-Moesin (phospho T558) antibody [EPR2371(2)] (ab177943) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : 293T cell lysate, treated with Calyculin
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling Moesin with ab177943 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Moesin with ab177943 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab177943 has been referenced in 6 publications.
- Luo S et al. Activation of TMEM16A Ca2+-activated Cl- channels by ROCK1/moesin promotes breast cancer metastasis. J Adv Res 33:253-264 (2021). PubMed: 34603794
- Qin Y et al. Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer. Sci Adv 6:eaaw9960 (2020). PubMed: 32128390
- Chen L et al. Advanced glycation end products induce immature angiogenesis in in vivo and ex vivo mouse models. Am J Physiol Heart Circ Physiol 318:H519-H533 (2020). PubMed: 31922896
- Hu S et al. CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin. Cancer Cell Int 20:563 (2020). PubMed: 33292278
- Chen W et al. Role of TLR4-MAP4K4 signaling pathway in models of oxygen-induced retinopathy. FASEB J N/A:fj201801086RR (2018). PubMed: 30475644
- Moreno-Fortuny A et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). PubMed: 28923978