Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2371(2)] to Moesin (phospho T558) - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-Moesin (phospho T558) antibody [EPR2371(2)] - BSA and Azide free
See all Moesin primary antibodies
DescriptionRabbit monoclonal [EPR2371(2)] to Moesin (phospho T558) - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Moesin aa 550 to the C-terminus (phospho T558) (Cysteine residue). The exact sequence is proprietary.
Database link: P26038
- Calyculin treated 293T cell lysate; Human gastric adenocarcinoma and prostate tissues.
Ab246834 is the carrier-free version of ab177943. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab246834 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab246834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane.
Tissue specificityIn all tissues and cultured cells studied.
Sequence similaritiesContains 1 FERM domain.
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
- Information by UniProt
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
All lanes : Anti-Moesin (phospho T558) antibody [EPR2371(2)] (ab177943) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : 293T cell lysate, treated with Calyculin
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa
This data was developed using ab177943, the same antibody clone in a different buffer formulation.
This data was developed using ab177943, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling Moesin with ab177943 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using ab177943, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Moesin with ab177943 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246834 has not yet been referenced specifically in any publications.