Recombinant Anti-Moesin (phospho T558) antibody [EPR2371(2)] - BSA and Azide free (ab246834)
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- References
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Overview
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Product name
Anti-Moesin (phospho T558) antibody [EPR2371(2)] - BSA and Azide free
See all Moesin primary antibodies -
Description
Rabbit monoclonal [EPR2371(2)] to Moesin (phospho T558) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Moesin aa 550 to the C-terminus (phospho T558) (Cysteine residue). The exact sequence is proprietary.
Database link: P26038 -
Positive control
- Calyculin treated 293T cell lysate; Human gastric adenocarcinoma and prostate tissues.
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General notes
Ab246834 is the carrier-free version of ab177943. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab246834 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2371(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab246834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
Tissue specificity
In all tissues and cultured cells studied. -
Sequence similarities
Contains 1 FERM domain. -
Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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Database links
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: P26038 Human
- SwissProt: P26041 Mouse
- SwissProt: O35763 Rat
- Unigene: 87752 Human
see all -
Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Datasheets and documents
References
ab246834 has not yet been referenced specifically in any publications.
