Key features and details
- Rabbit polyclonal to MOK protein kinase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MOK protein kinase antibody
DescriptionRabbit polyclonal to MOK protein kinase
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Recombinant fragment, corresponding to a region within amino acids 123-393 of Human MOK protein kinase (Uniprot ID: Q9UQ07).
- A549 cells; Human SW480 xenograft tissue; 293T, A431, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionAble to phosphorylate several exogenous substrates and to undergo autophosphorylation.
Tissue specificityExpressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Antigen recognized by autologous cytolytic T lymphocytes antibody
- EC 22.214.171.124 antibody
- LE 9211 A antigen antibody
Anti-MOK protein kinase antibody (ab154759) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
Immunofluorescent analysis of methanol-fixed A549 cells labeling MOK protein kinase with ab154759 at 1/100 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labeling MOK protein kinase with ab154759 at 1/100 dilution.
ab154759 has not yet been referenced specifically in any publications.