Product nameMonensin Solution (1,000X)
Formulation: Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell culture medium.
Concentration: 2.0 mM
Storage & Handling: Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact. Ethanol is flammable; keep away from sources of fire.
Application: ICFC - Quality tested
Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.
Monensin is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Monensin useful in intracellular staining of cytokines. Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
Tested applicationsSuitable for: Flow Cytmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 ml Monensin Solution (1,000X) 1 x 1ml
Our Abpromise guarantee covers the use of ab193381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
1/1000. Dilute from 1000X to 1X for use in tissue culture medium
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab193381 has not yet been referenced specifically in any publications.