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Formulation: Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell culture medium.
Concentration: 2.0 mM
Storage & Handling: Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact. Ethanol is flammable; keep away from sources of fire.
Application: ICFC - Quality tested
Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.
Monensin is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Monensin useful in intracellular staining of cytokines. Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
|Monensin Solution (1,000X)||1 x 1ml|
Our Abpromise guarantee covers the use of ab193381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
1/1000. Dilute from 1000X to 1X for use in tissue culture medium
ab193381 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"