Overview

  • Product name

    Monkey Cystatin C ELISA Kit
    See all Cystatin C kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum < 10%
    Inter-assay
    Sample n Mean SD CV%
    Serum < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.33 ng/ml
  • Range

    0.125 ng/ml - 4 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Monkey
  • Product overview

    Abcam’s Cystatin C Monkey ELISA Kit (ab190542) is for the measurement of Cystatin C in monkey serum and plasma.

    In this assay the Cystatin C present in samples reacts with the anti-Cystatin C antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Notes

    Cystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.

    The calibrator used in this kit is of human origin.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Components 1 x 96 tests
    1X Diluent Concentrate 1 x 60ml
    20X Wash Buffer Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Cystatin C Monkey Antibody coated microwells 1 x 96 tests
    Cystatin C Monkey Calibrator (lyophilized) 1 vial
    Cystatin C Monkey Detection Antibody 1 vial
    Cystatin C Monkey HRP Streptavidin Conjugate 1 vial
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Tissue specificity

    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • Involvement in disease

    Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.
    Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities

    Belongs to the cystatin family.
  • Post-translational
    modifications

    The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ARMD11
    • bA218C14.4 (cystatin C)
    • Cst3
    • Cystatin-3
    • Cystatin-C
    • CYTC_HUMAN
    • Gamma-trace
    • Neuroendocrine basic polypeptide
    • Post-gamma-globulin
    see all

Applications

Our Abpromise guarantee covers the use of ab190542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using Cystatin Monkey ELISA kit (ab190542)

Protocols

References

ab190542 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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