Overview

  • Product name

    Monkey IgG ELISA Kit
    See all IgG kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    IgG < 10%
    Inter-assay
    Sample n Mean SD CV%
    IgG < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.894 ng/ml
  • Range

    7.8 ng/ml - 500 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Monkey
  • Product overview

    Abcam’s IgG Monkey ELISA Kit kit (ab190549) is a two-site enzyme linked immunoassay (ELISA) for the measurement of IgG in monkey biological samples.

    In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Notes

    The calibrator used in this kit is of human origin. It is quantitated with purified Cynomolgus monkey IgG and behaves identically with it.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    IgG Monkey Antibody coated microwells 1 unit
    IgG Monkey Calibrator 1 vial
    IgG Monkey HRP Conjugate 1 vial
    Stop Solution 1 x 12ml
  • Research areas

  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Images

  • Representative standard curve using ab190549 IgG Monkey ELISA Kit

Protocols

References

ab190549 has not yet been referenced specifically in any publications.

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