Monkey IL-8 ELISA Kit (ab242232)
- Datasheet
- References
- Protocols
Overview
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Product name
Monkey IL-8 ELISA Kit
See all IL-8 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.1% Inter-assay Sample n Mean SD CV% serum 3 2.5% -
Sample type
Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.3 pg/ml -
Range
4.7 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 90% - 98% Serum 96 92% - 101% Cell culture media 112 107% - 121% Heparin Plasma 102 92% - 114% EDTA Plasma 82 76% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Cynomolgus monkey, Rhesus monkey -
Product overview
IL-8 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 protein in monkey serum, plasma, and cell culture supernatants.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml 10X Monkey IL-8 Capture Antibody 1 x 600µl 10X Monkey IL-8 Detector Antibody 1 x 600µl Monkey IL-8 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- (Ala-IL-8)77
- (Ser-IL-8)72
- 3 10C
see all -
Database links
- Entrez Gene: 3576 Human
- Entrez Gene: 613028 Rhesus monkey
- Omim: 146930 Human
- SwissProt: P10145 Human
- SwissProt: P67813 Rhesus monkey
- Unigene: 624 Human
- Unigene: 3363 Rhesus monkey
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab242232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Monkey IL-8 ELISA Kit (ab242232)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of monkey IL-8 standard curve in Sample Diluent NS.The IL-8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IL-8 in monkey serum and plasma samples.The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 2,072 pg/mL in neat serum, 644.2 pg/mL in neat plasma (heparin), and 248.7 pg/mL in neat plasma (EDTA). -
Interpolated concentrations of native IL-8 in stimulated and unstimulated Rhesus macaque peripheral blood mononuclear cell (PBMC) supernatant samples.Rhesus macaque PBMCs were cultured in the presence (stimulated) or absence (unstimulated) of 50 ng/mL PMA and 1 µg/mL ionomycin for 24 hours. The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 3%, and unstimulated PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 9,926 pg/mL in neat stimulated PBMC cell culture supernatant and 36.36 pg/mL in neat unstimulated PBMC cell culture supernatant. IL-6 was undetectable in naive cell culture media (not shown).
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Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours).The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2) and also shown in the table below.
Protocols
References
ab242232 has not yet been referenced specifically in any publications.
