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    monkey-il-8-elisa-kit-ab242232.pdf

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RecombinantSimpleStep

Monkey IL-8 ELISA Kit (ab242232)

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Other - Monkey IL-8 ELISA Kit (ab242232)
  • Example of monkey IL-8 standard curve in Sample Diluent NS.
  • Interpolated concentrations of native IL-8 in monkey serum and plasma samples.
  • Interpolated concentrations of native IL-8 in stimulated and unstimulated Rhesus macaque peripheral blood mononuclear cell (PBMC) supernatant samples.
  • Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours).
  • Sandwich ELISA - Monkey IL-8 ELISA Kit (ab242232)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.3 pg/ml
  • Range: 4.7 pg/ml - 300 pg/ml
  • Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey

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Overview

  • Product name

    Monkey IL-8 ELISA Kit
    See all IL-8 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.1%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 2.5%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.3 pg/ml
  • Range

    4.7 pg/ml - 300 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95 90% - 98%
    Serum 96 92% - 101%
    Cell culture media 112 107% - 121%
    Hep Plasma 102 92% - 114%
    EDTA Plasma 82 76% - 91%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey
  • Product overview

    Monkey IL-8 ELISA Kit (ab242232) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-8 protein in cell culture supernatant, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-8 with 2.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Monkey IL-8 Capture Antibody 1 x 600µl
    10X Monkey IL-8 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Monkey IL-8 Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Interleukin
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Interleukin ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Neuroscience
    • Processes
  • Function

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P10145 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 9E3
    • Beta thromboglobulin like protein
    • C-X-C motif chemokine 8
    • CEF-4
    • chemokine, CXC motif, ligand 8
    • CXCL8
    • Emoctakin
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Inteleukin 8
    • LECT
    • LUCT
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB 8
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG 1
    • TSG1
    see all

Associated products

  • Alternative Versions

    • Monkey IL-8 Antibody Pair - BSA and Azide free (ab256609)

Images

  • Other - Monkey IL-8 ELISA Kit (ab242232)
    Other - Monkey IL-8 ELISA Kit (ab242232)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of monkey IL-8 standard curve in Sample Diluent NS.
    Example of monkey IL-8 standard curve in Sample Diluent NS.
    The IL-8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native IL-8 in monkey serum and plasma samples.
    Interpolated concentrations of native IL-8 in monkey serum and plasma samples.
    The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 2,072 pg/mL in neat serum, 644.2 pg/mL in neat plasma (heparin), and 248.7 pg/mL in neat plasma (EDTA).
  • Interpolated concentrations of native IL-8 in stimulated and unstimulated Rhesus macaque peripheral blood mononuclear cell (PBMC) supernatant samples.
    Interpolated concentrations of native IL-8 in stimulated and unstimulated Rhesus macaque peripheral blood mononuclear cell (PBMC) supernatant samples.

    Rhesus macaque PBMCs were cultured in the presence (stimulated) or absence (unstimulated) of 50 ng/mL PMA and 1 µg/mL ionomycin for 24 hours. The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 3%, and unstimulated PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 9,926 pg/mL in neat stimulated PBMC cell culture supernatant and 36.36 pg/mL in neat unstimulated PBMC cell culture supernatant. IL-6 was undetectable in naive cell culture media (not shown).

  • Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours).
    Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours).
    The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2) and also shown in the table below.
  • Sandwich ELISA - Monkey IL-8 ELISA Kit (ab242232)
    Sandwich ELISA - Monkey IL-8 ELISA Kit (ab242232)
    To learn more about the advantages of recombinant antibodies see here.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab242232? Please let us know so that we can cite the reference in this datasheet.

ab242232 has not yet been referenced specifically in any publications.

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