Monkey MIP-1 alpha/CCL3 ELISA Kit (ab289698)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.7 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey MIP-1 alpha/CCL3 ELISA Kit
See all MIP-1 alpha/CCL3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 4.7% Inter-assay Sample n Mean SD CV% Supernatant 3 10.3% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 97% - 103% Serum 78 78% - 79% EDTA Plasma 101 97% - 108% Cit plasma 82 80% - 85% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Monkey
Does not react with: Mouse, Rat, Cow, Human -
Product overview
Monkey MIP-1 aplha/CCL3) ELISA Kit (ab289698) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey MIP-1 alpha/CCL3 in serum, plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey MIP-1 alpha/CCL3 with 8.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Monkey MIP-1 alpha/CCL3 Capture Antibody 1 x 600µl 10X Monkey MIP-1 alpha/CCL3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Monkey MIP-1 alpha/CCL3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine 3
- CCL 3
- CCL3
see all
Images
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Interpolated concentrations of monkey MIP-1 alpha in PBMC stimulated cell culture supernatant.
Interpolated concentration of native MIP-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples are 1:200 PBMC stimulated cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was observed in unstimulated.
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Example of monkey MIP-1 alpha standard curve in Sample Diluent NS.Example of monkey MIP-1 alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Monkey MIP-1 alpha/CCL3 ELISA Kit (ab289698) -
Monkey MIP-1 alpha/CCL3 ELISA Kit (ab289698)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289698 has not yet been referenced specifically in any publications.