Key features and details
- One-wash 90 minute protocol
- Sensitivity: 933.4 pg/ml
- Range: 1250 pg/ml - 80000 pg/ml
- Sample type: Hep Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Product nameMonkey Myoglobin ELISA Kit
See all Myoglobin kits
Intra-assay Sample n Mean SD CV% Plasma 8 7.3% Inter-assay Sample n Mean SD CV% Plasma 3 8.3%
Sample typeHep Plasma
Assay typeSandwich (quantitative)
Range1250 pg/ml - 80000 pg/ml
Sample specific recovery Sample type Average % Range Hep Plasma 106 96% - 119%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Monkey
Monkey Myoglobin ELISA Kit (ab269549) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Myoglobin with 933.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Myoglobin, a heme protein is found in both cardiac and skeletal muscle and functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Monkey Myoglobin Capture Antibody 1 x 600µl 10X Monkey Myoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Monkey Myoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionServes as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Sequence similaritiesBelongs to the globin family.
- Information by UniProt
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 218.62 ng/mL in plasma (heparin).
To learn more about the advantages of recombinant antibodies see here.
ab269549 has not yet been referenced specifically in any publications.