Anti-mono methyl Arginine antibody [5D1] (ab415)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-mono methyl Arginine antibody [5D1]
See all mono methyl Arginine primary antibodies -
DescriptionMouse monoclonal [5D1] to mono methyl Arginine
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Host speciesMouse
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Tested applicationsSuitable for: WB, ChIPmore details
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Species reactivityReacts with: Species independent
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General notes
This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
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Concentration information loading... -
PurityProtein A purified
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Primary antibody notesThis antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
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ClonalityMonoclonal
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Clone number5D1
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Myelomaunknown
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. | |
| ChIP | Use 5µl for 106 cells. |
Target
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Alternative names
- Arginine (mono methyl) antibody
Images
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![ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)](https://www.abcam.com/ps/products/0/ab415/Images/ab415_1.jpg)
ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UKSonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.
Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5
µ l of ab415 and 4x106 cells were used in each ChIP experiment. -
![ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)](https://www.abcam.com/ps/products/0/ab415/Images/ab415_2.jpg)
ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.
Protocols
References
This product has been referenced in:
- Slade DJ et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). Read more (PubMed: 23576281) »
- Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). Read more (PubMed: 17303166) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"