Anti-mono methyl Arginine antibody [5D1] (ab415)
Key features and details
- Mouse monoclonal [5D1] to mono methyl Arginine
- Suitable for: WB, ChIP
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-mono methyl Arginine antibody [5D1]
See all mono methyl Arginine primary antibodies -
Description
Mouse monoclonal [5D1] to mono methyl Arginine -
Host species
Mouse -
Tested applications
Suitable for: WB, ChIPmore details -
Species reactivity
Reacts with: Species independent -
General notes
This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status. -
Clonality
Monoclonal -
Clone number
5D1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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ChIP |
Use 5µl for 106 cells.
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Notes |
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WB
Use at an assay dependent dilution. |
ChIP
Use 5µl for 106 cells. |
Target
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Alternative names
- Arginine (mono methyl) antibody
Images
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Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.
Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5
µ l of ab415 and 4x106 cells were used in each ChIP experiment. -
We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab415 has been referenced in 7 publications.
- Jeong A et al. PRMT7 Inhibitor SGC8158 Enhances Doxorubicin-Induced DNA Damage and Its Cytotoxicity. Int J Mol Sci 23:N/A (2022). PubMed: 36293180
- Wimmers F et al. The single-cell epigenomic and transcriptional landscape of immunity to influenza vaccination. Cell 184:3915-3935.e21 (2021). PubMed: 34174187
- Liu Y et al. Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling. Elife 9:N/A (2020). PubMed: 32844749
- Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell 173:1385-1397.e14 (2018). PubMed: 29706550
- Slade DJ et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). PubMed: 23576281
- Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). PubMed: 17303166
- Cuthbert GL et al. Histone deimination antagonizes arginine methylation. Cell 118:545-53 (2004). PubMed: 15339660