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    mono-methyl-arginine-antibody-5d1-ab415.pdf

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Epigenetics and Nuclear Signaling Histones H3 Methylated
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Anti-mono methyl Arginine antibody [5D1] (ab415)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (4)

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ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)

Key features and details

  • Mouse monoclonal [5D1] to mono methyl Arginine
  • Suitable for: WB, ChIP
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-mono methyl Arginine antibody [5D1]
    See all mono methyl Arginine primary antibodies
  • Description

    Mouse monoclonal [5D1] to mono methyl Arginine
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ChIPmore details
  • Species reactivity

    Reacts with: Species independent
  • General notes

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
  • Clonality

    Monoclonal
  • Clone number

    5D1
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution.
ChIP
Use 5µl for 106 cells.
Notes
WB
Use at an assay dependent dilution.
ChIP
Use 5µl for 106 cells.

Target

  • Alternative names

    • Arginine (mono methyl) antibody

Images

  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
    ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.

    Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab415 and 4x106 cells were used in each ChIP experiment.

  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
    ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)

    We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.

     

Protocols

  • ChIP protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab415? Please let us know so that we can cite the reference in this datasheet.

    ab415 has been referenced in 4 publications.

    • Cheung P  et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell 173:1385-1397.e14 (2018). PubMed: 29706550
    • Slade DJ  et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). PubMed: 23576281
    • Raijmakers R  et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). PubMed: 17303166
    • Cuthbert GL  et al. Histone deimination antagonizes arginine methylation. Cell 118:545-53 (2004). PubMed: 15339660

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary antibody (recommended dilution is 1/10,000 to 1/50,000)

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2011

    Answer

    Thank you for your phone call. I am glad to hear that for ab109451 the protocol tips did work. I am very sorry that unfortunately, for ab413 and ab415, the tips did not improve your results. However, the details provided enable us to closely monitor the quality of our products. I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements with the order number xxx for ab107495 and ab107514. You should receive both vials tomorrow. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2011

    Question

    samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary antibody (recommended dilution is 1/10,000 to 1/50,000)

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2011

    Answer

    Thank you for your phone call. I am sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. As we discussed over the phone, I would like to offer some suggestions to help optimize the results: For ab413, ab415 I'd suggest using less primary and/or secondary antibody as the membrane shouldn't turn black. This might be an indication of too much antibody on the membrane. For ab109451, most likely using less primary antibody could help with the background as the recommended dilution for the antibody is 1/10,000 to 1/50,000. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Abcam Scientific Support

    Answered on Nov 22 2011

    Question

    Concerning ab415, ab414, ab413 - says whole antiserum; is it tisue culture supernatant or ascites? Do ab412 and ab413 cross-reat with symmetrical Ng/Na - dimethyl arginine?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2004

    Answer

    Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

    Read More

    Abcam Scientific Support

    Answered on Apr 28 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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