Anti-Monoacylglycerol Lipase/MGL antibody (ab228598)
Key features and details
- Rabbit polyclonal to Monoacylglycerol Lipase/MGL
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Monoacylglycerol Lipase/MGL antibody
See all Monoacylglycerol Lipase/MGL primary antibodies -
Description
Rabbit polyclonal to Monoacylglycerol Lipase/MGL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment within Human Monoacylglycerol Lipase/MGL (internal sequence). The exact sequence is proprietary.
Database link: Q99685 -
Positive control
- WB: Mouse white adipose tissue lysate; HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Mouse esophagus tissue; rat fore brain tissue; U-373 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
Target
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Function
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
Tissue specificity
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
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Database links
- Entrez Gene: 505290 Cow
- Entrez Gene: 11343 Human
- Entrez Gene: 23945 Mouse
- Entrez Gene: 29254 Rat
- Omim: 609699 Human
- SwissProt: Q99685 Human
- SwissProt: O35678 Mouse
- SwissProt: Q8R431 Rat
see all -
Alternative names
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
Images
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Paraffin-embedded mouse esophagus tissue stained for Monoacylglycerol Lipase/MGL with ab228598 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Monoacylglycerol Lipase/MGL antibody (ab228598) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa10% SDS-PAGE
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Paraffin-embedded rat forebrain tissue stained for Monoacylglycerol Lipase/MGL with ab228598 at 1/500 dilution in immunohistochemical analysis.
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Anti-Monoacylglycerol Lipase/MGL antibody (ab228598) at 1/1000 dilution + Mouse white adipose tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa10% SDS-PAGE
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Paraffin-embedded U-373 (human) xenograft tissue stained for Monoacylglycerol Lipase/MGL with ab228598 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab228598 has been referenced in 1 publication.
- Wang C et al. Inhibition of monoacylglycerol lipase restrains proliferation, migration, invasion, tumor growth and induces apoptosis in cervical cancer. J Obstet Gynaecol Res 48:456-466 (2022). PubMed: 34877750