Recombinant Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free (ab248009)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6339] to Monoacylglycerol Lipase/MGL - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free
See all Monoacylglycerol Lipase/MGL primary antibodies -
Description
Rabbit monoclonal [EPR6339] to Monoacylglycerol Lipase/MGL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248009 is the carrier-free version of ab124796 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248009 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Monoacylglycerol Lipase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6339 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
Target
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Function
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
Tissue specificity
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
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Database links
- Entrez Gene: 11343 Human
- Omim: 609699 Human
- SwissProt: Q99685 Human
- Unigene: 277035 Human
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Alternative names
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
Images
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Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (ab124796) at 1/1000 dilution + Human adipose tissue lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDaThis data was developed using ab124796, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab248009 has not yet been referenced specifically in any publications.