Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)

Overview

  • Product name

    Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101]
    See all Monoamine Oxidase A/MAO-A primary antibodies
  • Description

    Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Monoamine Oxidase A/MAO-A aa 450-550. The exact sequence is proprietary.
    (Peptide available as ab196045)

  • Positive control

    • HepG2, NCI-H460 and fetal liver lysates; Human colon and kidney tissues
  • General notes

    Previously labelled as Monoamine Oxidase A. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
    • Tissue specificity

      Heart, liver, duodenum, blood vessels and kidney.
    • Involvement in disease

      Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior.
    • Sequence similarities

      Belongs to the flavin monoamine oxidase family.
    • Cellular localization

      Mitochondrion outer membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amine oxidase [flavin containing] A antibody
      • Amine oxidase [flavin-containing] A antibody
      • AOFA antibody
      • AOFA_HUMAN antibody
      • EC 1.4.3.4 antibody
      • MAO A antibody
      • MAO-A antibody
      • maoA antibody
      • Monoamine oxidase A antibody
      • Monoamine oxidase type A antibody
      see all

    Images

    • Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/2000 dilution + Mouse brain cortex tissue lysate - whole at 20 µg

      Secondary
      HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 60 kDa


      Exposure time: 1 minute

      See Abreview

    • All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : MAOA (Monoamine Oxidase A) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 60 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab126751 observed at 60 kDa. Red - loading control, ab8245, observed at 0 kDa.

      ab126751 was shown to recognize Monoamine Oxidase A in wild-type HAP1 cells as signal was lost at the expected MW in MAOA (Monoamine Oxidase A) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MAOA (Monoamine Oxidase A) knockout samples were subjected to SDS-PAGE. Ab126751 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab126751, at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human colon tissue by Immunohistochemistry.

    • ab126751 staining Monoamine Oxidase A/MAO-A in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : NCI-H460 lysate
      Lane 3 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    • ab126751, at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human kidney tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Liu CZ  et al. Rasagiline, an inhibitor of MAO-B, decreases colonic motility through elevating colonic dopamine content. Neurogastroenterol Motil N/A:e13390 (2018). Read more (PubMed: 29956417) »
    • Matthews BA  et al. Elevated monoamine oxidase A activity and protein levels in rodent brain during acute withdrawal after chronic intermittent ethanol vapor exposure. Drug Alcohol Depend 185:398-405 (2018). WB ; Rat . Read more (PubMed: 29549852) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain cortex)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    Brain cortex
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Sep 24 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain, skin, small and large intestines)
    Permeabilization
    No
    Specification
    Brain, skin, small and large intestines
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Jul 06 2013

    Answer

    Thank you for contacting us. The antibody has not been tested in frozen sections but I would expect it to work. Typically antibodies tested in paraffin sections do work in frozen sections, although the reverse is not necessarily true. We do put on the datasheet if an application is unsuitable, otherwise it has not been tested. If you do wish to use these products in IHC-Fr, we do have a program called the Abtrial which gives you a discount off a future purchase for testing and reviewing a product in an application we have not tested. Please let me know if you are interested in this program.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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