Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)

Reviews (2)Q&A (1)References (18)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101]
    See all Monoamine Oxidase A/MAO-A primary antibodies

  • Description

    Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Monoamine Oxidase A/MAO-A aa 450-550. The exact sequence is proprietary.
    (Peptide available as ab196045)

  • Positive control

    • WB: HepG2, Rat brain, and Mouse brain lysates. IHC-P: human hepatocellular carcinoma, mouse liver, and rat liver tissues. ICC/IF: HepG2 cells.

  • General notes

    Previously labelled as Monoamine Oxidase A. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab126751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

  • Tissue specificity

    Heart, liver, duodenum, blood vessels and kidney.

  • Involvement in disease

    Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior.

  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.

  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Amine oxidase [flavin containing] A antibody
    • Amine oxidase [flavin-containing] A antibody
    • AOFA antibody
    • AOFA_HUMAN antibody
    • EC 1.4.3.4 antibody
    • MAO A antibody
    • MAO-A antibody
    • maoA antibody
    • Monoamine oxidase A antibody
    • Monoamine oxidase type A antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:100 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MAOA (Monoamine Oxidase A) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 60 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab126751 observed at 60 kDa. Red - loading control, ab8245, observed at 0 kDa.

    ab126751 was shown to recognize Monoamine Oxidase A in wild-type HAP1 cells as signal was lost at the expected MW in MAOA (Monoamine Oxidase A) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MAOA (Monoamine Oxidase A) knockout samples were subjected to SDS-PAGE. Ab126751 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/10000 dilution (Purified) + Mouse brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

  • Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

  • Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)This image is courtesy of an anonymous Abreview
    Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/2000 dilution (unpurified) + Mouse brain cortex tissue lysate - whole at 20 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 1 minute

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)

    ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Flow Cytometry - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)

    ab126751 (purified) staining Monoamine Oxidase A/MAO-A in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 lysate
    Lane 2 : NCI-H460 lysate
    Lane 3 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)

    ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Other - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Other - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

Publishing research using ab126751? Please let us know so that we can cite the reference in this datasheet.

ab126751 has been referenced in 18 publications.

  • Konar A  et al. Brain region specific methylation and Sirt1 binding changes in MAOA promoter is associated with sexual dimorphism in early life stress induced aggressive behavior. Neurochem Int 129:104510 (2019). PubMed: 31348967
  • Scalabrin M  et al. Redox responses in skeletal muscle following denervation. Redox Biol 26:101294 (2019). PubMed: 31450104
  • Gwynne WD  et al. Monoamine oxidase-A activity is required for clonal tumorsphere formation by human breast tumor cells. Cell Mol Biol Lett 24:59 (2019). PubMed: 31754354
  • Guo D  et al. Tyrosine hydroxylase down-regulation after loss of Abelson helper integration site 1 (AHI1) promotes depression via the circadian clock pathway in mice. J Biol Chem 293:5090-5101 (2018). PubMed: 29449373
  • Cha YJ  et al. Site-specific expression of amine oxidases in breast cancer metastases. Tumour Biol 40:1010428318776822 (2018). PubMed: 29775160
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain cortex)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Sep 24 2013

Immunohistochemistry (Frozen sections) abreview for Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101]

 Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain, skin, small and large intestines)
Permeabilization
No
Specification
Brain, skin, small and large intestines
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Jul 06 2013

Question

Dear Abcam,



I am interested in the anti-monoamine oxidase A (ab126751) and anti-monoamine oxidase B (ab125010) RabMAbs antibodies from your company. However, I would be using them for immunohistochemistry with frozen tissues, and the applications for the two antibodies listed on your website is for IHC-P only. I am just wondering if there would be a problem for using these antibodies on frozen tissues (i.e. due to changes in antigenic site), or if the application is not listed because it has not be validated yet.

Read More
Answer

Thank you for contacting us. The antibody has not been tested in frozen sections but I would expect it to work. Typically antibodies tested in paraffin sections do work in frozen sections, although the reverse is not necessarily true. We do put on the datasheet if an application is unsuitable, otherwise it has not been tested. If you do wish to use these products in IHC-Fr, we do have a program called the Abtrial which gives you a discount off a future purchase for testing and reviewing a product in an application we have not tested. Please let me know if you are interested in this program.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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