Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)

Overview

  • Product name

    Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free
    See all Monoamine Oxidase A/MAO-A primary antibodies
  • Description

    Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Monoamine Oxidase A/MAO-A aa 450-550. The exact sequence is proprietary.
    (Peptide available as ab196045)

  • General notes

    Ab240031 is the carrier-free version of ab126751. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240031 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
    • Tissue specificity

      Heart, liver, duodenum, blood vessels and kidney.
    • Involvement in disease

      Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior.
    • Sequence similarities

      Belongs to the flavin monoamine oxidase family.
    • Cellular localization

      Mitochondrion outer membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amine oxidase [flavin containing] A antibody
      • Amine oxidase [flavin-containing] A antibody
      • AOFA antibody
      • AOFA_HUMAN antibody
      • EC 1.4.3.4 antibody
      • MAO A antibody
      • MAO-A antibody
      • maoA antibody
      • Monoamine oxidase A antibody
      • Monoamine oxidase type A antibody
      see all

    Images

    • All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : MAOA (Monoamine Oxidase A) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 60 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab126751 observed at 60 kDa. Red - loading control, ab8245, observed at 38 kDa.

      ab126751 was shown to recognize Monoamine Oxidase A in wild-type HAP1 cells as signal was lost at the expected MW in MAOA (Monoamine Oxidase A) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MAOA (Monoamine Oxidase A) knockout samples were subjected to SDS-PAGE. Ab126751 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).

    • ab126751 staining Monoamine Oxidase A/MAO-A in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).

    • ab126751, at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human colon tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).

    • ab126751, at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human kidney tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).

    References

    ab240031 has not yet been referenced specifically in any publications.

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