Question (22484) | Monoamine oxidase B Activity Assay Kit ((MAOB Assay) (ab109912)

Go to datasheet (ab109912)

Question

What was the immunogen used to raise this antibody in this kit? Do you expect this kit to cross-react with pig?

Answer

Thank you for your inquiry. These antibodies were generated using the shotgun immunization approach so we do not know the exact epitope. There was no peptide sequence used to immunize so unfortunately I cannot BLAST an immunogen sequence with pig. However I can say that the activity assay for MAOB is bovine (cow) crossreactive as tested by the laboratory (not shown on datasheet). Furthermore human, cow and pig have close similarity (91% identical in primary sequence).  Therefore, this assay has a good chance of working with pig but we wouldn't be able to guarantee it since we haven't yet tested this kit in pig. I hope this information helps. Please contact us with any other questions.