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What would the manufacturer recommend that a customer use for a standard for the activity and the quantity of the enzyme? Do they (or you) know of a quantified recombinant MAO-B enzyme that could be used for both activity and quantity? Have they tried using a recombinant for this purpose?
Asked on Jul 22 2011
Thank you for your reply. I forwarded your question to the lab at Mitosciences, and they are not aware of a source of the active MAOB enzyme so they have not tested the kit with a recombinant standard. They recommend generating a standard curve with normal sample, demonstrated in the protocol. The data is generally reported as activity per mg or ug of normal sample. I hope this information is helpful, but please let me know if you have any further questions.
Answered on Jul 22 2011