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我公司客户购买了Abcam公司货号为ab109912的Monoamine oxidase B (MAOB) Specific Activity Assay Kit，在实验过程中遇到以下问题，请帮忙解决！
We ordered a Monoamine Oxidase B Enzyme Specific Activity Microplate Assay Kit to determine the MAOB level in the serum samples.
Now, we have some questions on the performance of the kit as following:
1. How to perform the quantity analysis without the reference in the kit?
2. For the serum samples, will the pre-treatment be needed?
Asked on Apr 23 2012
Thank you for your inquiry.
1. I can confirm that this assay does not deliver the result asa quantitative amount of MAOB. This kit can be used to quantitatively measure the different between two samples.
For example. Sample 1 is untreated and samples 2 is treated. the difference in MAOB can be measured.
If you would like to create your own standard curve, I suggest to MAOB in defined amounts.
2. Unfortunately, this kit is not tested and guaranteed for serum samples. I suggest to prepare a serial dilution of samples with assay buffer. This has to be optimised and is not guaranteed.
For more information please see the protocol booklet.
I am sorry I did not have positive answer to your questions on this occasion and wish you good luck with your research.
Answered on Apr 23 2012