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We ordered a Monoamine Oxidase B Enzyme Specific Activity Microplate Assay Kit to determine the MAOB level in the serum samples. Now, we have some questions on the performance of the kit as following:
1. How to perform the quantity analysis without the reference in the kit?
2. For the serum samples, will the pre-treatment be needed?
Asked on Apr 24 2012
Thank you for your enquiry and your interest in our products.
The Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912) has been designed for cell culture extracts.
We would recommend using this kit in ‘profiling’ assay which means it is to be used when comparing the levels of protein in two samples – normal and unknown/treated.
We currently don’t have access to an acceptable AMOB protein that is sufficiently native and works in the kit. As soon as we have further information, we will update the on-line product datasheet accordingly.
For your interest there is also a MAOB activity/quantity assay ab109912 in our catalogue that may be of interest.
I hope this helps and if I can assist further, please do not hesitate to contact me.
Answered on Apr 24 2012