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Thanks for your reply.
We will try to use this kit to assay serum samples and hope we can get some positive results. But I am a little confused about the method.
Now we have some samples collected from different time points. We will consider the samples before dose as normal samples and the samples after dose as treated samples, respectively.
Can we compare the MAOB level of treated samples with that of the normal samples at the same time point by directly loading these samples on the microplate and reading the response. I think that will be a qualitative assay, not a quantity assay. How do you think about it?Thanks ,
Asked on Apr 25 2012
Thank you for your response.
It is important to emphasize that as the datasheet suggests this kit has been designed and tested on cell culture extracts but not specifically on serum samples. As long as MAOB (in serum) is detectable, you could make comparison between control samples and treated samples at different time points and/or at different concentrations.
If you need any further assistance, please do not hesitate to contact me.
Answered on Apr 25 2012