Overview

  • Product name
    Anti-Monoamine Oxidase B antibody
    See all Monoamine Oxidase B primary antibodies
  • Description
    Goat polyclonal to Monoamine Oxidase B
  • Host species
    Goat
  • Specificity
    This antibody is not expected to cross-react with Monoamine oxidase A.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-HKARKLARLTKEE

    , corresponding to internal sequence amino acids 347-359 of Human Monoamine Oxidase B

  • Positive control
    • Human Brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 21873469

Target

Images

  • All lanes : Anti-Monoamine Oxidase B antibody (ab47988) at 1 µg/ml

    Lane 1 : Human Brain lysate (35µg protein in RIPA buffer) with no blocking with the immunizing peptide
    Lane 2 : Human Brain lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide

    Predicted band size: 59 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-Monoamine Oxidase B antibody (ab47988) at 0.5 µg/ml + Human brain (hippocampus) lysate in RIPA buffer. at 35 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Francis H  et al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut 61:753-64 (2012). ICC/IF ; Human . Read more (PubMed: 21873469) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for your inquiry. Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. The protocol is as follows: ELISA: 1.) Coat 0.1ug of peptide in 100ul PBS to micro-wells of high binding ELISA plates (Greiner) overnight at 4C. 2.) Wash three times with 300uL of PBS 3.) Block with PBS/ 0.1% (v/v) Tween-20 (PBS-T) with 3% (w/v) skimmed milk for 1h at 37C. 4.) Wash three times with 300uL of PBS-T 5.) Incubate 100ul serially diluted antibody in blocking buffer in the peptide-coated microwells at 37C with the plates covered for 1h. 6.) Wash three times with 300uL of PBS-T 7.) Incubate with AP-conjugated secondary antibody in blocking buffer. 8.) Wash four times with 300uL PBS-T followed by two washes with 300uL PBS. 9.) Develop with 50ul p-nitro phenyl phosphate (pNPP) at 30C for one hour and stop development by adding 50ul 1M NaOH. 10.) Read at 405nm. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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