Overview

  • Product name

    Anti-Monoamine Oxidase B/MAOB antibody
    See all Monoamine Oxidase B/MAOB primary antibodies
  • Description

    Mouse polyclonal to Monoamine Oxidase B/MAOB
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Common marmoset
  • Immunogen

    Full length protein corresponding to Human Monoamine Oxidase B/MAOB.
    Database link: NP_000889.3

  • Positive control

    • Human liver lysate. Human kidney tissue. heLa cells.
  • General notes

     This product was previously labelled as Monoamine Oxidase B

     

Properties

Applications

Our Abpromise guarantee covers the use of ab88510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
    • Sequence similarities

      Belongs to the flavin monoamine oxidase family.
    • Cellular localization

      Mitochondrion outer membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adrenalin oxidase antibody
      • Amine oxidase (flavin containing) antibody
      • Amine oxidase [flavin-containing] B antibody
      • AOFB_HUMAN antibody
      • MAO, brain antibody
      • MAO, platelet antibody
      • MAO-B antibody
      • MAOB antibody
      • MGC26382 antibody
      • Monoamine oxidase B antibody
      • Monoamine oxidase type B antibody
      • OTTHUMP00000023166 antibody
      • Tyramine oxidase antibody
      see all

    Images

    • Anti-Monoamine Oxidase B/MAOB antibody (ab88510) at 1 µg/ml + Human liver lysate at 50 µg

      Predicted band size: 59 kDa
      Observed band size: 59 kDa

    • All lanes : Anti-Monoamine Oxidase B/MAOB antibody (ab88510) at 1 µg/ml

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 59 kDa
      Observed band size: 59 kDa

    • Purified ab88510 at 3ug/ml staining Monoamine Oxidase B/MAOB in formalin-fixed, paraffin embedded Human kidney tissue.

    • Immunofluorescent staining of Monoamine Oxidase B/MAOB in HeLa cells using purified ab88510 at 10ug/ml.

    References

    This product has been referenced in:

    • Grouzmann E  et al. Monoamine oxidase a down-regulation contributes to high metanephrine concentration in pheochromocytoma. J Clin Endocrinol Metab 97:2773-81 (2012). Human . Read more (PubMed: 22569243) »
    • Peritore CS  et al. Resveratrol attenuates L-DOPA-induced hydrogen peroxide toxicity in neuronal cells. Neuroreport 23:989-94 (2012). Read more (PubMed: 23114623) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Common marmoset Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Loading amount
    25 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jun 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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