Recombinant Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24131-79] to Monoamine Oxidase B/MAOB
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79]
See all Monoamine Oxidase B/MAOB primary antibodies -
Description
Rabbit monoclonal [EPR24131-79] to Monoamine Oxidase B/MAOB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and liver tissue lysates; Rat brain and liver tissue lysate; E.coli extracts containing his-tagged mouse Amine oxidase [flavin-containing] B recombinant protein (aa 2-489). IHC-P: Mouse liver; Rat liver. IP: Mouse liver tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24131-79 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 59 kDa.
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| IP |
1/30.
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 59 kDa. |
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IP
1/30. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine. -
Sequence similarities
Belongs to the flavin monoamine oxidase family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 109731 Mouse
- Entrez Gene: 25750 Rat
- SwissProt: Q8BW75 Mouse
- SwissProt: P19643 Rat
- Unigene: 241656 Mouse
- Unigene: 6656 Rat
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Alternative names
- Adrenalin oxidase antibody
- Amine oxidase (flavin containing) antibody
- Amine oxidase [flavin-containing] B antibody
see all
Images
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Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab259928)All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 59 kDa
Observed band size: 59 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 8058968).
Low expression: mouse testis.
Protein aggregates may be caused by boiling of samples .
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259928)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on mouse liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab259928)All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution
Lane 1 : E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi B recombinant protein (aa 2-489) at 10 µl
Lane 2 : E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi A recombinant protein (aa 1-497) at 4 µl
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 59 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259928)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on rat liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunoprecipitation - (ab259928)Monoamine Oxidase B was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259928 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259928 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate 10 ug
Lane 2: ab259928 IP in Mouse liver tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259928 in mouse liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
Sample loaded onto lane 1 are non-boiled as boiling may cause protein aggregates.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab259928 has not yet been referenced specifically in any publications.