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  1. Link

    monoamine-oxidase-bmaob-antibody-epr7102-ab133270.pdf

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RecombinantRabMAb

Recombinant Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

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Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102]
    See all Monoamine Oxidase B/MAOB primary antibodies
  • Description

    Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Monoamine Oxidase B/MAOB aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, fetal kidney, platelet, fetal brain and SH SY5Y lysates; Human brain and kidney tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.90 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7102
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Monoamine Oxidase B/MAOB protein (ab82944)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol

Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
  • Cellular localization

    Mitochondrion outer membrane.
  • Target information above from: UniProt accession P27338 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4129 Human
    • Omim: 309860 Human
    • SwissProt: P27338 Human
    • Unigene: 654473 Human
    • Alternative names

      • Adrenalin oxidase antibody
      • Amine oxidase (flavin containing) antibody
      • Amine oxidase [flavin-containing] B antibody
      • AOFB_HUMAN antibody
      • MAO, brain antibody
      • MAO, platelet antibody
      • MAO-B antibody
      • MAOB antibody
      • MGC26382 antibody
      • Monoamine oxidase B antibody
      • Monoamine oxidase type B antibody
      • OTTHUMP00000023166 antibody
      • Tyramine oxidase antibody
      see all

    Images

    • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 59 kDa
      Observed band size: 59 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/2000 dilution (purified)

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 59 kDa
      Observed band size: 59 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Immunohistochemical staining of paraffin embedded human liver with purified ab133270 at a working dilution of 1/300. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/1000 dilution (Unpurified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human platelet lysate
      Lane 4 : Human fetal brain lysate
      Lane 5 : SH SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 59 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

      Immunohistochemical analysis of paraffin embedded human brain tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100Produced under U.S. Patent No. 5,675,063.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

      Immunohistochemical analysis of paraffin embedded human kidney tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100 dilution.

    • OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
      Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab133270? Please let us know so that we can cite the reference in this datasheet.

    ab133270 has not yet been referenced specifically in any publications.

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