Recombinant Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
![Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-257455-anti-monoamine-oxidase-b-antibody-epr7102-western-blot.jpg "Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Monoamine Oxidase B/MAOB antibody [EPR7102]
See all Monoamine Oxidase B/MAOB primary antibodies -
Description
Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Monoamine Oxidase B/MAOB aa 1-100. The exact sequence is proprietary.
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Positive control
- Human fetal liver, fetal kidney, platelet, fetal brain and SH SY5Y lysates; Human brain and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.90 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7102 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
Target
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Function
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine. -
Sequence similarities
Belongs to the flavin monoamine oxidase family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 4129 Human
- Omim: 309860 Human
- SwissProt: P27338 Human
- Unigene: 654473 Human
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Alternative names
- Adrenalin oxidase antibody
- Amine oxidase (flavin containing) antibody
- Amine oxidase [flavin-containing] B antibody
see all
Images
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Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-257454-anti-monoamine-oxidase-b-antibody-epr7102-western-blot.jpg)
Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/2000 dilution (purified)
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-257427-anti-monoamine-oxidase-b-antibody-epr7102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)Immunohistochemical staining of paraffin embedded human liver with purified ab133270 at a working dilution of 1/300. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-146718-anti-monoamine-oxidase-b-antibody-epr7102-western-blot.jpg)
Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/1000 dilution (Unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human platelet lysate
Lane 4 : Human fetal brain lysate
Lane 5 : SH SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-146717-anti-monoamine-oxidase-b-antibody-epr7102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)Immunohistochemical analysis of paraffin embedded human brain tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100Produced under U.S. Patent No. 5,675,063.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-146716-anti-monoamine-oxidase-b-antibody-epr7102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)Immunohistochemical analysis of paraffin embedded human kidney tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100 dilution.
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![OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-146715-anti-monoamine-oxidase-b-antibody-epr7102.jpg)
OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) -
![Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)](https://www.abcam.com/ps/products/133/ab133270/Images/ab133270-8-benefits-of-recombinant-antibodies.png)
Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133270 has not yet been referenced specifically in any publications.