Recombinant
RabMAb

Recombinant Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Submit a review Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102]
    See all Monoamine Oxidase B/MAOB primary antibodies

  • Description

    Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Monoamine Oxidase B/MAOB aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, fetal kidney, platelet, fetal brain and SH SY5Y lysates; Human brain and kidney tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Monoamine Oxidase B

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab133270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.

    • Sequence similarities

      Belongs to the flavin monoamine oxidase family.

    • Cellular localization

      Mitochondrion outer membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Adrenalin oxidase antibody
        • Amine oxidase (flavin containing) antibody
        • Amine oxidase [flavin-containing] B antibody
        • AOFB_HUMAN antibody
        • MAO, brain antibody
        • MAO, platelet antibody
        • MAO-B antibody
        • MAOB antibody
        • MGC26382 antibody
        • Monoamine oxidase B antibody
        • Monoamine oxidase type B antibody
        • OTTHUMP00000023166 antibody
        • Tyramine oxidase antibody
        see all

      Images

      • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

        Secondary
        Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

        Predicted band size: 59 kDa
        Observed band size: 59 kDa



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/2000 dilution (purified)

        Lane 1 : Human fetal kidney lysate
        Lane 2 : Human fetal brain lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

        Predicted band size: 59 kDa
        Observed band size: 59 kDa



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Immunohistochemical staining of paraffin embedded human liver with purified ab133270 at a working dilution of 1/300. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
      • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/1000 dilution (Unpurified)

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human fetal kidney lysate
        Lane 3 : Human platelet lysate
        Lane 4 : Human fetal brain lysate
        Lane 5 : SH SY5Y cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 59 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

        Immunohistochemical analysis of paraffin embedded human brain tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100Produced under U.S. Patent No. 5,675,063.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

        Immunohistochemical analysis of paraffin embedded human kidney tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100 dilution.

      • Other - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Other - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      Publishing research using ab133270? Please let us know so that we can cite the reference in this datasheet.

      ab133270 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab133270.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com