Monoamine Oxidase (MAO) Assay Kit (ab241031)

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Overview

  • Product name

    Monoamine Oxidase (MAO) Assay Kit

  • Detection method

    Fluorescent

  • Sample type

    Serum, Plasma

  • Product overview

    Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively. The assay is based on the fluorometric detection of H2O2, one of the by-products generated during the oxidative deamination of the MAO substrate (Tyramine). The assay can detect as little as 5 µU of MAO enzymatic activity.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Developer (Lyophilized) 1 vial
    H2O2 Standard (0.88 M) 1 x 100µl
    MAO Assay Buffer 1 x 25ml
    MAO Substrate (Lyophilized) 1 vial
    MAO-A Inhibitor, Clorgyline (Lyophilized) 1 vial
    MAO-B Inhibitor, Selegiline (Lyophilized) 1 vial
    Positive Control (MAO-A Enzyme) (Lyophilized) 1 vial
    Probe (in DMSO) 1 x 220µl

  • Research areas

  • Relevance

    Monoamine oxidases are enzymes that catalyze the oxidation of monoamines. They belong to protein family of flavin containing amine oxidoreductases. In humans there are two types of MAO: MAO-A and MAO-B. Both are found in neurons and astroglia. Outside the central nervous system: MAO-A is also found in the liver, gastrointestinal tract and placenta. MAO-B is mostly found in blood platelets. Because of the vital role that MAOs play in the inactivation of neurotransmitters, MAO dysfunction (too much/too little MAO activity) is thought to be responsible for a number of neurological disorders.

  • Cellular localization

    Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.

  • Alternative names

    • Adrenalin oxidase
    • Amine oxidase
    • Flavin containing A
    • Flavin containing B
    • MAO brain
    • MAO platelet
    • Monoamine oxidase A
    • Monoamine oxidase B
    • Monoamine oxidase type A
    • Monoamine oxidase type B
    • Tyramine oxidase
    see all

Images

  • H2O2 Standard Curve.
    H2O2 Standard Curve.

    H2O2 Standard Curve.

  • MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg)
    MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg)

    MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg).

  • Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.
    Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.
    Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.

Protocols

References

ab241031 has not yet been referenced specifically in any publications.

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