Overview

  • Product name
    Anti-Monocyte + Macrophage antibody [MOMA-2]
  • Description
    Rat monoclonal [MOMA-2] to Monocyte + Macrophage
  • Host species
    Rat
  • Specificity
    This antibody recognises an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs in all mouse strains tested. Staining may also be seen on the epithelium of mucosa.
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse lymph node stroma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    MOMA-2
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24449804
IHC-FoFr 1/50.
Flow Cyt Use at an assay dependent concentration.

Membrane permeabilisation is required for this application.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/25.

Target

  • Relevance
    Monocyte and macrophage are white blood cells that roam the body tissues engulfing foreign organisms. A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Macrophages are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defense (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.
  • Alternative names
    • Macrophages antibody
    • Monocytes antibody

Images

  • ab33451 staining Monocyte + Macrophage in mouse aorta tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 5% serum for 30 minutes at room temperature. The primary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour. A FITC-conjugated rabbit anti-rat polyclonal was used as the secondary antibody, diluted 1/400.

    Endothelium is stained with Von Willebrand Factor (ab6994) and counterstained with DAPI.

    See Abreview

  • ab33451 staining mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 18 hours at 4°C.  A HRP conjugated goat anti-rat antibody, diluted 1/500, was used as the secondary. 

    This antibody labels perivascular (left) and subpial (right) macs in normal brain tissue.

    See Abreview

References

This product has been referenced in:
  • Jung YY  et al. Atherosclerosis is exacerbated by chitinase-3-like-1 in amyloid precursor protein transgenic mice. Theranostics 8:749-766 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29344304) »
  • Zeng X  et al. Contribution of TLR4 signaling in intermittent hypoxia-mediated atherosclerosis progression. J Transl Med 16:106 (2018). Read more (PubMed: 29673358) »
See all 33 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us.

ab7815 and ab33451 have been tested with Frozen sections only and we do not have any empirical data to support their performance with paraffin tissues.

I have found the following ICAM1 and Monocyte + macrophage antibodies which have been tested in IHC-P and might be of your interest.

Anti-ICAM1 antibody:

ab2213 ab109361 ab33894. They are not biotin labelled.

Anti-Monocyte + Macrophage antibody


ab51934. React with human.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

The following reference appears to be the source of the statement on the ab33451 datasheet (anti-macrophage/monocyte, MOMA-2 clone) regarding reactivity with mucosa epithelium:

The localization of macrophage subsets and dendritic cells in the gastrointestinal tract of the mouse with special reference to the presence of high endothelial venules. An immuno- and enzyme-histochemical study. Cell Tissue Res. 1990 Mar;259(3):587-93. PMID: 2180576

http://www.ncbi.nlm.nih.gov/pubmed/2180576

However, I was not able to access the article to confirm. The same laboratory developed the MOMA-2 clone, described in the following reference:

Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study. Scand J Immunol. 1987 Dec;26(6):653-61. PMID: 2180576

http://www.ncbi.nlm.nih.gov/pubmed/3321409

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your phone call.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test abab33451 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Pand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 08 2011

Answer

Thanks so much for keeping me updated and letting me know how the replacement antibody worked. I am so happy to hear that the replacement antibody ab53444 worked in your staining! Perhaps the non-specific staining could be reduced by increasing the blocking time or lowering the secondary antibody concentration. If this background is problematic then I would be happy to help you further. Thanks again for the update, and let me know if you have any questions or need anything else from me. Have a great week!

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Question
Answer

Thanks for your call today and for letting us know about the trouble with ab33451. I am sorry to hear that this antibody did not produce the expected results. As we discussed, I am sending a free of charge vial of ab53444 on the order ***, which I expect to arrive tomorrow. Please keep me updated about the results using this antibody, and let me know if there is anything else that we can do for you.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Aorta)
Specification
Aorta
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (normal brain)
Specification
normal brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
TNB as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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