Key features and details
- Rabbit polyclonal to Morg1 - Aminoterminal end
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Morg1 antibody - Aminoterminal end
DescriptionRabbit polyclonal to Morg1 - Aminoterminal end
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to a sequence near the N-terminus of human Morg1, identical to the related mouse and rat sequence.
FormLyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
FunctionMolecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator (By similarity). May also be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the WD repeat MORG1 family.
Contains 7 WD repeats.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Partially nuclear.
- Information by UniProt
- MAPK organizer 1 antibody
- MGC4238 antibody
- Mitogen activated protein kinase organizer 1 antibody
This image shows human mammary cancer tissue stained with ab65870 at 1µg/ml. Cytoplasmic staining of Morg1, using DAB.
Anti-Morg1 antibody - Aminoterminal end (ab65870) at 1 µg/ml + rat brain tissue lysate
Predicted band size: 34 kDa
Observed band size: 34 kDa
ICC/IF image of ab65870 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65870, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab65870 has been referenced in 1 publication.
- Su WY et al. Bidirectional regulation between WDR83 and its natural antisense transcript DHPS in gastric cancer. Cell Res : (2012). ICC/IF . PubMed: 22491477