Overview

Properties

  • Form

    Liquid
  • Storage instructions

    Store at room temperature.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Reagent notes

    ab64230 is formulated for coverslipping sections. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • ab64230 Mounting Medium used along with anti-CD20 antibody on Human Tonsil

References

This product has been referenced in:

  • Chen YC  et al. Laser-emission imaging of nuclear biomarkers for high-contrast cancer screening and immunodiagnosis. Nat Biomed Eng 1:724-735 (2017). Read more (PubMed: 29204310) »
  • Nakamura F  et al. Documentation and localization of force-mediated filamin A domain perturbations in moving cells. Nat Commun 5:4656 (2014). Read more (PubMed: 25120197) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for replying with that information.

As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)for IHC on fresh PBMC samples, asallof these kits have only been tested on formalin fixed paraffin embedded tissue samples.Wewould expect that as long as the primary antibody binds the target as expected that both kits could be suitable foryour samples but as we have not tested it ourselves we would not be able to guarentee this.



There should be good contrast if using the AEC or DAB kit with hematoxylin as counterstain. We counterstain with hematoxylin in-house regardless ofwhether DAB or AEC is used. The sensitivity betweenAEC and DABis similar. I would recommend using ab94361 or ab80437 if you expect the PBMC samples to contain any endogenous biotin.I am sorry but I cannot recommend a particular supplier of hematoxylin.

And to answer your final question, the mounting media ab64230 should be suitable for your purposes.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

Thank you for contacting us.

Differences in the chemical compositions of the mounting medium make using one product for both IHC and IF difficult. The conditions required for excellent perioxidase preservation are not neccessarily good for the anti- photobleaching requirements of IF. As such I cannot recommend a single medium for you customer but suggest they by mounting mediums specific to their needs.

Our standard mointing medium (ab64230;https://www.abcam.com/Mounting-Medium-ab64230.html) is appropriate for IHC.



For IF I recommend BrightMount/Plus (ab103748;https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html)


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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