Overview

  • Product name

    Mounting Medium With DAPI - Aqueous, Fluoroshield
    See all Fluoroshield reagents
  • Description

    Mounting Medium With DAPI
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-Pmore details
  • General notes

    Mounting Medium with DAPI ab104139 (previously called Fluoroshield Mounting Medium With DAPI) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. It is fortified with DAPI as a counter-stain for DNA (DAPI also stains RNA).

    The formulation prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, R-Phycoerythrin (R-PE), Phyocyanin (PC), and Allophycocyanin (APC).

    Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.


    Mounting media products

    For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium ab104135Mounting Medium with PI ab104129, and this product, Mounting Medium with DAPI ab104139. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

    For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141

     

    Procedure

    - Bring the vial to room temperature.

    - Rinse slide to be mounted with distilled or deionized water, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.

    - Turn the vial upside down and open the dropper to remove any air bubbles.

    - Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.

    - Let stand at room temperature for about 5 minutes.

    - Apply cover slip carefully avoiding air bubbles.

    - The specimen is ready for visualization under a microscope.

    Seal the edges of cover slip with nail polish, any organic medium or Limonene mounting medium ab104141. If a coverslip is not sealed, air bubbles will appear in a few days.

    Store the slide in the dark at 2-8°C.

    Removal of Coverslip
    Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

Properties

Applications

Our Abpromise guarantee covers the use of ab104139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Images

  • Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI (ab104139).

    See Abreview

  • ab104139 worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus.

References

This product has been referenced in:

  • Xu XY  et al. Role of the P2X7 receptor in inflammation-mediated changes in the osteogenesis of periodontal ligament stem cells. Cell Death Dis 10:20 (2019). Read more (PubMed: 30622236) »
  • Muraleva NA  et al. p38 MAPK-dependent alphaB-crystallin phosphorylation in Alzheimer's disease-like pathology in OXYS rats. Exp Gerontol 119:45-52 (2019). Read more (PubMed: 30664924) »
See all 87 Publications for this product

Customer reviews and Q&As

Filter by Ratings

1-7 of 7 Abreviews

tried many different product to get nuclear staining better and more clear with mounting solution but failed then I bought abcam ab104139 - Fluoroshield Mounting Medium With DAPI in 3T3L1 adipocyte cells as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best product to detect nuclear staining with mounting solution during in vivo and in vitro studies

Abcam user community

Verified customer

Submitted Aug 17 2018

Best quality-price ratio

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've tried some others, but I'll definitively stick with this one. Nice stain and affordable price.

Abcam user community

Verified customer

Submitted Aug 31 2017

Excellent and convenient

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've used a number of various mounting media - SouthernBiotech, Invitrogen/Life Tech, Vectashield, and now this. This one is my favorite for several reasons: (1) Affordability, (2) Packaging, and (3) ease of use.

Abcam user community

Verified customer

Submitted Jul 31 2017

Good Solution for fast Staining

Excellent Excellent 5/5 (Ease of Use)
Abreviews
It works as expected. Picture shows an example of DAPI positive nuclei.

Abcam user community

Verified customer

Submitted Aug 19 2016

Works great!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This dye works great for frozen or paraffin embedded skin tissue in all our experiments.

Ahmar Aziz

Verified customer

Submitted May 05 2016

Excellent results

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI.

Mr. Bryan Niedenberger

Verified customer

Submitted Mar 09 2016

Fantastic!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I was worried about some of the reviews but the price made it an easy decision. I was pretty happy since it worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus

Sebastian Alvarado

Verified customer

Submitted Dec 08 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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